Chapter 6. DNA Microarray Analysis

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Animation Activity 6-26: DNA Microarray Analysis

1.

________ can be used to probe the mRNA content of cells and determine which genes are expressed under a given condition.

PCR

Oligonucleotide arrays

Gene-expression patterns

dNTPs

Fluorophores

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2.

You use an oligonucleotide array to analyze the gene expression patterns in two types of human cancer cells, melanoma and breast cancer. How do you know which hybridizations on the array correspond to each cancer type?

A scanner detects patterns of hybridization by sensing specific cDNA libraries.

The cDNAs of each cell type are fluorescently labeled with different colored fluorophores and detected by the scanner.

Melanoma cells are much larger than breast cancer cells, so size distinguishes them.

You have a predetermined list of where each will hybridize.

The oligonucleotides from each cell type cannot hybridize to the same sequence.

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3.

Put the following steps for screening an oligonucleotide array for patterns of gene expression in the correct order:
1. The activated genes in each sample are identified.
2. A scanner detects patterns of hybridization.
3. Pools of cDNAs are incubated with the oligonucleotide array under hybridization conditions.
4. Total mRNA is isolated from each sample to be analyzed.
5. The mRNAs are converted to fluorescently labeled cDNAs.

5, 4, 3, 2, 1

5, 3, 2, 1, 4

4, 3, 2, 1, 5

4, 5, 3, 2, 1

1, 3, 2, 4, 5

Correct.

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Chapter 6. DNA Microarray Analysis

Introduction

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