Chapter 6. Protein-Fusion Using Green Fluorescent Protein

Animation Activities

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Animation Activity 6-31: Protein-Fusion Using Green Fluorescent Protein

A reporter construct in which the green fluorescent protein (GFP) gene is fused to gene X is best used to ________.

quantify the normal expression of Protein X in cells

stimulate association of Protein X with the nuclear envelope

activate Protein X enzyme activity

knock down expression of endogenous Protein X in a cell

visualize the subcellular localization of the Protein X-GFP chimeric protein

Correct.

Incorrect.

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A GFP reporter construct can be used to determine which cells in an organism express Protein X by fusing the GFP-encoding gene to ________.

the Protein X-encoding sequence (gene X)

the promoter region of gene X

an ER signal sequence

a sequence encoding a protein that interacts with Protein X

Any of the above would work.

Correct.

Incorrect.

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Which of the following is important for the design and use of a reporter construct for investigating the subcellular localization of Protein X?

The reporter gene sequence and gene X are fused in the same reading frame.

The construct containing gene X and the reporter gene can be introduced into cells.

Both of the above are important.

Correct.

Incorrect.

Try Again.

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Introduction