Chapter 6. Genome Editing Using CRISPR-Cas9

Animation Activities

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Animation Activity 6-43: Genome Editing Using CRISPR-Cas9

Genome editing via the CRISPR-Cas9 system can result in:

single nucleotide mutations.

frameshift mutations.

nonhomologous end joining.

homology-directed repair.

All of the above are correct.

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The CRISPR-Cas9 system consists of all of the following EXCEPT:

target sequence construct.

chimeric guide RNA.

Cas9 gene construct.

RNA polymerase.

TracrRNA scaffold sequence.

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What is a difference between the two CRISPR-Cas9 editing mechanisms: homology-directed repair and nonhomologous end joining?

creation of a double-stranded break in the target sequence in homology-directed repair

cleavage of the target gene by Cas9 in nonhomologous end joining

introduction of a homologous DNA segment to the target gene in homology-directed repair

use of a guide RNA complementary to the sequence of the target gene in nonhomologous end joining

All of the above are correct.

Correct.

Incorrect.

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Introduction