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Mechanism of Rearrangement of Immunoglobulin Gene Segments via Deletional Joining

Chapter 23. Mechanism of Rearrangement of Immunoglobulin Gene Segments via Deletional Joining

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Animation Activity 23-16: Mechanism of Rearrangement of Immunoglobulin Gene Segments via Deletional Joining

1.

The conserved sequences present between the different coding regions within the heavy and light chain loci of immunoglobulin genes that are critical for recombination are called:

V segments.

J segments.

D segments.

intervening DNA sequences.

recombination signal sequences (RSS).

Correct.

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2.

Somatic recombination of immunoglobulin genes is catalyzed by:

terminal deoxynucleotidyl transferase (TdT).

DNA ligase 4.

RAG recombinases.

DNA polymerase.

exonucleases.

Correct.

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3.

Terminal deoxynucleotidyl transferase (TdT) catalyzes which of the following reactions?

cleavage of a single strand of the DNA between the RSS and coding region

formation of a covalently closed hairpin structure from the cleaved DNA coding regions

formation of an asymmetric cleavage in the hairpin structure, leaving a palindromic overhang

addition of nucleotides in a template-independent manner to the ends of cleaved hairpin sequences

All of the above

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4.

Without the exonuclease activity that occurs during the final steps of the immunoglobulin gene recombination reaction, what would be expected?

The N regions would not be added to the cleaved ends of the opened hairpins.

The binding between complementary N region sequences would be prevented.

The unpaired nucleotides between two complementary N region sequences would not be removed.

Cleavage between the coding segment and the RSS would not occur.

The sequence gaps between two paired complementary N regions would not be filled in with nucleotides.

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Chapter 23. Mechanism of Rearrangement of Immunoglobulin Gene Segments via Deletional Joining

Introduction

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