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Question 1 of 4
Animation Assessment
Question
Put the following immunoblotting steps in the correct order. 1-Chromogenic reaction to detect bound antibody 2-Secondary antibody binds the primary antibody 3-Primary antibody binds the protein of interest 4-Blocking of the nitrocellulose membrane to prevent non-specific protein binding 5-Transfer of proteins from the gel to the blot using electrophoresis
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Question
When using the immunoblotting technique, the primary antibody detects
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2
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Question
When using the immunoblotting technique, the secondary antibody detects
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2
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Question
Enzyme linked secondary antibodies allow detection of
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2
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