Screening an Oligonucleotide Array for Patterns of Gene Expression
Chapter 5. Screening an Oligonucleotide Array for Patterns of Gene Expression
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____________________ can be used to probe the mRNA content of cells and determine which genes are expressed under a given condition.
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You use an oligonucleotide array to analyze the gene expression patterns in two types of human cancer cells, melanoma and breast cancer. How do you know which hybridizations on the array correspond to each cancer type?
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Put the following steps for screening an oligonucleotide array for patterns of gene expression in the correct order: 1- Activated genes are identified. 2- A scanner detects patterns of hybridization. 3- Pools of cDNAs are incubated with the oligonucleotide array under hybridization conditions. 4- Total mRNA is isolated from each cell type to be analyzed. 5- The mRNAs are converted to fluorescently-labeled cDNAs.
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