Chapter 7. Yeast Two-Hybrid System

In the Yeast Two Hybrid system used to identify unknown binding partners for a bait protein, expression of which gene directly reports interaction of a fish protein hybrid with the bait protein hybrid?

In the first step of the two-step selection process, growth of transfected yeast cells on media that contains histidine but lacks tryptophan and leucine demonstrates that each yeast cell:

Fish proteins that are encoded in a cDNA library and that interact with a bait protein in yeast growing on –Trp, -Leu, -His plates can be identified most easily by: