image
FIG. 5.8
Photo source: FRAP of cytoplasmic EGFP in living HeLa cells, performed using an UltraVIEW® spinning disk confocal system (PerkinElmer Inc.). HeLa cells were transfected with pEGFP-C1 (Clontech Laboratories, Inc.) using GeneJuice® transfection reagent (Novagen®). A region of interest in the cytoplasm was photobleached using the UltraVIEW® photokinesis unit, and the recovery of fluorescence in this region was observed. (Fluorescence Recovery After Photobleaching (FRAP) using the UltraVIEW PhotoKinesis accessory, PerkinElmer Technical Note).