Chapter 16. Using Algae to Treat Toxins

Using Algae to Treat Toxins

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Professor Mike Cohen of Sonoma State University has built a series of algae ponds to treat toxins. He and his students test the rate of water flow, the kind of algae and aquatic plants being used, and water purity.

Contaminants down your drain

Without even knowing it, many people flush toxic products down their drains. Along with the soapy water, wastewater treatment plants receive cleaning chemicals, cosmetic ingredients, and even estrogens from the birth control pills women take (which pass through into their urine). The toxic residue of pharmaceutical products ends up at treatment plants as well when clueless people put pills down their drains.

This is why researchers are studying algae. Professor Mike Cohen of Sonoma State University has constructed trial ponds that use algae and other aquatic plants to purify the water of stubborn chemicals. Built at the City of Santa Rosa Laguna Wastewater Treatment Plant in California, the ponds take in water that has been through the normal treatment process, and scrub away many remaining pollutants.

Building an algae filter at a treatment plant

At the Santa Rosa Laguna Wastewater Treatment Plant, sewage water is stirred through murky pools. Aerating bubbles take ammonia, which comes from the urea in urine, and turn it into nitrate. Bacteria then turn nitrates into nitrogen gas. Pools like these do an effective job of getting out human waste, but flushed contaminants that are small, stubborn, or very concentrated can escape the purification process. As a result, after the treated water is released into rivers and streams, it may contain ingredients that harm fish and wildlife.

Wild filamentous algae (pictured) are preferred to other types in this filtration method.

To make an algae filter, Cohen and his students collect samples of wild algae and aquatic plants from natural ponds. Cohen says that lush, wild filamentous algae (the kind that grow on the surface of the water) are better filters than planktonic algae (the kind that sometimes find their way into these pools on their own and cloud the water).

Cohen and his students grow the wild algae in shallow pools at the wastewater treatment facility. After the water has been through the treatment process, it’s pumped to the algae channel, where the green slime scrubs the water as it grows and multiplies.

How algae clean up toxins

Much like humans digest sugar, some algae use toxins as a source of carbon by breaking down the toxic chemicals. The presence of algae and plants can also stimulate photodegredation. This means the toxins are broken down by light energy, which is transferred from the algae molecules to toxins, such as pharmaceutical wastes. Filamentous algae additionally support the growth of bacteria, which consume nutrients from organic pollutants. There are more of these good bacteria in algae ponds than in typical water treatment pools.

Cohen and his students found that diverting water through constructed algae channels greatly reduces the amount of estrogen compounds, nitrates, and metals. Many of the toxins missed during the wastewater treatment process are removed. When the water is finally returned to the environment, it’s less harmful to fish and wildlife.

Treating eutrophication

This same technology can be used to purify rivers, lakes, and streams that suffer increased nutrient levels due to pollution—a condition called eutrophication. Nitrogen compounds from agricultural and lawn fertilizers seep into waterways when it rains. This runoff over-fertilizes ecosystems, resulting in rampant weed growth. Residual waste products from the agricultural industry have been linked to clogged wetlands and rivers, which fill up quickly with non-native trees and shrubs. When these pollutants reach large bodies of water, they cause toxic algal blooms that kill fish.

By diverting some of the water in a stream through a constructed canal of harmless algae, the nitrites are almost entirely removed. The algae use these nutrients as food, returning the water to the stream in a cleaner state.

Converting algae to fuel

At the Laguna Water Treatment Plant algae are digested onsite to make methane--used to run a generator, which charges electric vehicles at the plant.

Since the algae use the toxins for food, they are constantly growing and dividing. To keep water flowing through the ditches and ponds at the right rate, algae must be harvested. Fortunately, there are a few uses for the goop! Algae can be pressed to make biodiesel—an oily fuel that is burned in diesel cars in place of fossil fuel.

Algae can also be fermented in a digester to make methane—a clean-burning fuel that can be used to run an electricity generator. Cohen is doing this at the Laguna Water Treatment plant.

While algae filtration methods were first developed in the 1950s, most treatment plants opted to build standard facilities instead. Recent research has helped validate algae as a viable water scrubber. Now, waste treatment facilities in California, Texas, and other states are integrating algae beds and constructed wetlands to better purify water.

Although, not even the best algae pond can get rid of all pollutants. As very low doses of estrogenic and pharmaceutical compounds can have a big environmental impact, it’s best to keep these from going down the drain in the first place.

© 2015 WH Freeman and Company.

16.1 Comprehension Questions

Answer the following questions to demonstrate your understanding of the article.

Question 16.1

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
2
Correct. Algae that is harvested from water treatment facilities can be used to produce biodiesel. Using algae to power vehicles means not having to dispose of it in landfills.
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Incorrect. Algae that is harvested from water treatment facilities can be used to produce biodiesel. Using algae to power vehicles means not having to dispose of it in landfills.

Question 16.2

Harvested algae also can be fermented in a digester to create mQCND0zqtYCPAWjViecvOQs7XwQSVYoPcRVvjLrT0CpBaHpmLj+vkM1zssI=, a clean-burning fuel.

2
Right. Like biodiesel, methane is a fuel that can be produced from algae. As the article notes, Professor Cohen is using digested algae at the Laguna Water Treatment Plant to produce methane. The methane is fueling an electricity generator, which is used to power electric cars at the plant.
Try again. You have one attempt remaining.
Incorrect. Like biodiesel, methane is a fuel that can be produced from algae. As the article notes, Professor Cohen is using digested algae at the Laguna Water Treatment Plant to produce methane. The methane is fueling an electricity generator, which is used to power electric cars at the plant.

Question 16.3

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
2
Right. Bacteria converts nitrates into nitrogen. Nitrogen is nontoxic and makes up nearly 80% of the air we breathe.
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Incorrect. Bacteria converts nitrates into nitrogen. Nitrogen is nontoxic and makes up nearly 80% of the air we breathe.

Question 16.4

Fill in the Blank:

Fertilizer run-off from lawns and farmland can cause a condition known as YdCrFYGhMp8qaA7z1S09hlu6Jso=, which is defined as excessive nutrient levels in rivers, lakes, and streams as a result of pollution.

2
Correct. When fertilizers flow into bodies of water, they can increase growth of foreign plants and algae, which can clog waterways. Additionally, when plants and algae die and are decomposed by bacteria, dissolved oxygen is used up, killing fish and other marine animals.
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Incorrect. Run-off can cause eutrophication. When fertilizers flow into bodies of water, they can increase growth of foreign plants and algae, which can clog waterways. Additionally, when plants and algae die and are decomposed by bacteria, dissolved oxygen is used up, killing fish and other marine animals.

Question 16.5

Algae and plants can help stimulate photodegradation, where toxins are broken down by S9A45h4ztC/ZCB7AP7yrg8rZWhfoVkDzjuYlrj+Ws9Rp2J3l9cM3gg== energy.

2
Correct. Light energy, which is transferred from algae molecules, helps break down toxins such as pharmaceutical wastes.
Try again. You have one attempt remaining.
Incorrect. Light energy, which is transferred from algae molecules, helps break down toxins such as pharmaceutical wastes.

Question 16.6

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
2
Correct. Professor Cohen and his students found that this step of diverting wastewater through algae channels helped reduce the amount of estrogen compounds, nitrates, and metals that were missed during the wastewater treatment process, in turn preventing these substances from being released into the environment where they could harm wildlife.
Try again. You have one attempt remaining.
Incorrect. Professor Cohen and his students found that this step of diverting wastewater through algae channels helped reduce the amount of estrogen compounds, nitrates, and metals that were missed during the wastewater treatment process, in turn preventing these substances from being released into the environment where they could harm wildlife.

Question 16.7

True or False:

Professor Cohen and his students found that the best type of algae for “scrubbing” wastewater of particulate matter is planktonic algae.

IkLyhbbYRLnR0DDUHGa+YA==

Correct. This statement is false. According to Cohen and his students’ observations, the best type of algae for scrubbing wastewater of particulate matter is wild filamentous algae, which grows on the surface of water.
Incorrect. This statement is false. According to Cohen and his students’ observations, the best type of algae for scrubbing wastewater of particulate matter is wild filamentous algae, which grows on the surface of water.

Question 16.8

sVmIjvCLwMUn8X7qgg3HHQsthGa94NMvbJIpiLhXwuIcmfZ+HI4Y0xjyv/vTxgKI8TMS40hxMayJOF6D555ULTc2k2h6g/ldzYrzVpMywZpL4ghtNsD2hcT1C1x0UFvbwh1mXh3kEBN2ekZjtW5GzSK5AIts2N3nriJdpF0ZllRN+eGPB8PWbcobiR4xIOBCbeQhuGnmJ81CdylxdiwFm3EdAz2Py1v3KEMuwptrX2CUcRGtb1E/dq9yaj1cx9mht+3wZ4k01fV5N9QImYchQvJSL0J926VpmmmAVmnaPhNcZKp7SMIllj7KuxGreEZ+Apnx1g25IfG98zrXQvcDcm43uuwwELp/4tkFQjcQK3pIhQ3FTCHnz5l6vQJHYYEa/e5Qmr5de8JceNnhWEWWYhB1iS3u+mmhn/NiBZOQGSBkxz0ET+Fl4PU7l+KIFitW1i7uhbQxZ25Qrl6BVV77RYFmPfUldo++bHPK6vgsmjtWaDRRpE10ZM8xlLN6LgHm4GggbYbz8ahtXTu//+WZlMg0wLY/FGRGBskw1I5QQUqD0vIt6dwOS3ym0jdqnulJ+x+xfvb0UerBa+NAkEzrKeTt5y3LyT9FWnZLhUJ/bVZlG8OZhUXiRZPr62Skl8M6TJJ94SdmorTONfEahkkamAHivFqMGIdVe0H3HqUjYLRwMpajzYNy7JMbOcZ2KnaMgiDzGYGqKAWLvtCxDxk71V6HrZv0u+IMLzTdE3AJvGvhxlHtuk0paJuQuMic0PdE65WEl4GBCBrWOktTx9PxymElqywDDaH1L39hbyDF9b4dm59ARGhjEJQAbrQ62wjujrRV+25luhgR/oNLNTn6H6Q8WzyHEFzGEwo05smmwqyjZLoc1tTjEyuaQATVuCvGjjcmcoYbPTXjAMsXTwIYJHZbHE3asPKrRUyBeWvcXg9w0gQdOWsO/Y/CGwyQwCp0wEEIkbueLowxFNgzE8hy7H7/Ret0UCoDmvnX0WHcLIexgrym+xsFxUAXDwuuHe/3WK3tY4gOCZbBIlUXbu7fjpLu5H6CymVIgYW+hwZM4c+/6nOHvXsQw2NYRey4OaOzv0DBaR8aWYzSaofA4jEJV9WGT3vwJ6LG0tXwLNZS55cpEJLnB4ghj6XaFLnmD5pEnc3bFb1CLRGlfa8noJk3zPrEZeyPh/fpdhWx7PPXdtPESmnasPCU7jILqFa/cXuWHFQAxJ7rs+UMnv6S/4o5HHy78+TJEYTqgfvdhSvfRQBKfAD/1Li1uJG7y42Sz8ng++s1I8mDj0WJaLIvJH2V1cTSRQRVD5FJG1a3jMKHfXpFtPsEbJ3mSvSqBiGbdOEHiubhppa+itrpirzrGmP/NVgiiQuC2Ehqd9CTzUO+/Y7EBUmg2Q3VbF8UPBtzgaaimm07QGXxLKWsbj+f7dUuQluTJnU7hvXGeAMOXHUG2z31oKgq60ovQzEmb9Hs/XFfC1MyN0ABJV1tkiDZGGi8zdkk2WdcyZs5e56iyB8YXRkS4fFgItkiUcbLlR17yliMv29qMCqbqKBY+/uUnobzEInMuBW4uSHlP0UETleFwVXL2on7hIdSmoS0qtCEXjeUw83JkY06PeJ0WdjUlqm5latl+eTZr3hPTk3QYge0WtgAQEmKn9iSlcTchFq4hCyB+lEEzvft/J3Aw6qT/3u22LJ57ARX+jr5un0SPcd+0SD/4pzAShntN6fqiDWBgRiVKiUWYIXNz7eMNRAeehf8ecSfU221LGa9XZAXZxWkMjXcD5XvLM2P/VApMPwxdT314M0goaxh/FNuFBwHPhWTTNzjSPGf3Z3cEhH7uJ8795rdTaf7C056EmzvEW5LHC4U7pknNnRJW79E2fPIlK1yxXwf5tFYKa7dgkjiV4z9ZZB2AS3fIggg+yjHlRIz/2hMMxJGOFQZUDtNAien
2
Correct. As the article notes, many people pour medicines down the drain or flush them as a means of disposal. The problem is, pharmaceutical compounds often aren’t broken down during wastewater treatment and get released into the environment, where they can have dire effects on wildlife. Most pharmacies have ways to properly dispose of medications to prevent this from happening.
Try again. You have one attempt remaining.
Incorrect. Taking them to the pharmacy for disposal is the best option. As the article notes, many people pour medicines down the drain or flush them as a means of disposal. The problem is, pharmaceutical compounds often aren’t broken down during wastewater treatment and get released into the environment, where they can have dire effects on wildlife. Most pharmacies have ways to properly dispose of medications to prevent this from happening.

Read the following paragraph and refer to the figure when answering the next 2 questions.

The figure above is a simplified diagram of a wastewater treatment plant. (You can click on the image and expand it for closer examination.) Wastewater, including sewage and other wastes from homes and businesses, enters the plant through pipe A, and then moves into the screening tank (B). Here, large objects are filtered out of the wastewater. Next, wastewater leaves the screening tank and enters the aerating tank (C). The aerating tank uses bubbles of air to take ammonia from urea and converts the ammonia to nitrate. The tank also contains bacteria that break down the nitrates into nitrogen gas. Once bacteria break down the organic materials, wastewater then moves through a pipe (D) to the algae tank (E), where toxins and other hazardous materials are broken down. Next, the wastewater moves through a pipe (F) to the purifying tank (G), where living organisms (i.e. bacteria) are killed by chlorine. From there, the sanitized water is then pumped into a body of water—in this case, a river (H).

Question 16.9

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2
Correct. To determine the effectiveness of this wastewater treatment plant, it is necessary to determine the composition of wastewater at points throughout the entire system. First, you’d need to test water entering the system at pipe A. Next, it is important to measure organic materials leaving the aeration tank at pipe D to determine the aeration tank’s effectiveness. Finally, you would want to sample the wastewater as it leaves the algae tank on its way to the purifying tank to determine what toxins were removed by the algae. With this sampling strategy, it is possible to determine what is removed by the aeration tank and algae tank and make necessary adjustments to improve efficiency.
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Incorrect. To determine the effectiveness of this wastewater treatment plant, it is necessary to determine the composition of wastewater at points throughout the entire system. First, you’d need to test water entering the system at pipe A. Next, it is important to measure organic materials leaving the aeration tank at pipe D to determine the aeration tank’s effectiveness. Finally, you would want to sample the wastewater as it leaves the algae tank on its way to the purifying tank to determine what toxins were removed by the algae. With this sampling strategy, it is possible to determine what is removed by the aeration tank and algae tank and make necessary adjustments to improve efficiency.

Question 16.10

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2
Correct. Bubbles of air in the aerating tank typically take ammonia from urea and convert it to nitrate. In turn, bacteria then convert nitrates into nitrogen gas. If air stopped bubbling, urea would not be converted to nitrate, and bacteria—which are aerobic—would quickly use up oxygen needed for converting nitrates into nitrogen gas, and ultimately would die. If you took a sample from location “D,” you would find high levels of urea in the wastewater.
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Incorrect. Bubbles of air in the aerating tank typically take ammonia from urea and convert it to nitrate. In turn, bacteria then convert nitrates into nitrogen gas. If air stopped bubbling, urea would not be converted to nitrate, and bacteria—which are aerobic—would quickly use up oxygen needed for converting nitrates into nitrogen gas, and ultimately would die. If you took a sample from location “D,” you would find high levels of urea in the wastewater.

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