| Cloning Vector |
Size of DNA That Can Be Cloned |
Method of Propagation |
Introduction to Bacteria |
| Plasmid |
As large as 15 kb |
Plasmid replication |
Transformation |
| Phage lambda |
As large as 23 kb |
Phage reproduction |
Phage infection |
| Cosmid |
As large as 44 kb |
Plasmid reproduction |
Phage infection |
| Bacterial artificial chromosome |
As large as 300 kb |
Plasmid reproduction |
Electroporation |
| Note: 1 kb = 1000 bp. Electroporation consists of electrical pulses that increase the permeability of a membrane. |
Table 19.2: Comparison of plasmids, phage lambda vectors, cosmids, and bacterial artificial chromosomes