1. | Identify the nonrecombinant progeny (two most-numerous phenotypes). |
2. | Identify the double-crossover progeny (two least-numerous phenotypes). |
3. | Compare the phenotype of double-crossover progeny with the phenotype of nonrecombinant progeny. They should be alike in two characteristics and differ in one. |
4. | The characteristic that differs between the double crossover and the nonrecombinant progeny is encoded by the middle gene. |