Challenge Questions
Section 9.2
Question 9.42
As a summer project, a microbiology student independently isolates two mutations in E. coli that are auxotrophic for glycine (gly−). The student wants to know whether these two mutants are at the same functional unit. Outline a procedure that the student could use to determine whether these two gly− mutations occur within the same functional unit.
Question 9.43
A group of genetics students mix two auxotrophic strains of bacteria: one is leu+ trp+ his− met− and the other is leu− trp− his+ met+. After mixing the two strains, they plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). They assume that some gene transfer has taken place between the two strains. How can they determine whether the transfer of genes is due to conjugation, transduction, or transformation?
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