Chapter 9. Animation Activity: Bacterial Conjugation

After you've completed the activity, click the button below to answer questions about what you've learned.

Animation Assessment

Question

pdrQzDvIEwSmnBCpwWFxQpYCaRIj2CkZin5mgMgxdNQ274EjuY2XGDUmsIePDxCr4mr5Tv8DkBFAIX+k18iwU2q/Ggvk97iHlUiz6YCW6rqmofUGGZRxfW9ppCupmur4SwOJeRMCG4Vx6GrmAner1CDSFFD7XKIRwmw+Zo/WdRukZKtjssgImotPkzFf7s6KlecnJ+lUK0pqLkr51WXXdAHBIkVjHsipX/MkDh68mUY5fbdPaQijo0YF7LSsei5Dn5Z0DwbDzWV/n+bktY0pBw==
2
Correct. About 1 in 10,000 recipients of the F factor will integrate the F factor episome and become Hfr bacteria. Therefore, if there were 1 million recipients we would expect approximately 100 Hfr bacteria to result.
You have one more chance to answer the question correctly. Try again.
Incorrect. About 1 in 10,000 recipients of the F factor will integrate the F factor episome and become Hfr bacteria. Therefore, if there were 1 million recipients we would expect approximately 100 Hfr bacteria to result.

Question

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
2
Correct. In most cases the F- recipient, resulting from a conjugation with an Hfr donor, will remain F- because the entire F-factor is only rarely transferred. However, the bacterial chromosomal genes coming into the recipient provides the opportunity for recombination that may change the phenotype of the recipient F- bacterium.
You have one more chance to answer the question correctly. Try again.
Incorrect. In most cases the F- recipient, resulting from a conjugation with an Hfr donor, will remain F- because the entire F-factor is only rarely transferred. However, the bacterial chromosomal genes coming into the recipient provides the opportunity for recombination that may change the phenotype of the recipient F- bacterium.

Question

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
2
Correct. The lac+ gene from the Hfr bacteria will enter the F- bacteria as a linear piece of DNA which is unstable in bacteria. The lactose-metabolizing genes are perfectly active on a circular plasmid or on the bacterial chromosome. In most cases the F- bacteria will remain F- after an interrupted mating experiment.
You have one more chance to answer the question correctly. Try again.
Incorrect. The lac+ gene from the Hfr bacteria will enter the F- bacteria as a linear piece of DNA which is unstable in bacteria. The lactose-metabolizing genes are perfectly active on a circular plasmid or on the bacterial chromosome. In most cases the F- bacteria will remain F- after an interrupted mating experiment.

Question

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
2
Correct. Following the nick in the Hfr bacterium’s chromosome, the free 5’ end (leading strand) of the donor moves through the transfer pore into the F- recipient bacterium. As a result, those genes that follow the free 5’ end of the nicked DNA strand are more likely to be transferred than those near the 3’ end of the nick which would only enter the F- bacterium if the entire chromosome is transferred, which happens only rarely.
You have one more chance to answer the question correctly. Try again.
Incorrect. Following the nick in the Hfr bacterium’s chromosome, the free 5’ end (leading strand) of the donor moves through the transfer pore into the F- recipient bacterium. As a result, those genes that follow the free 5’ end of the nicked DNA strand are more likely to be transferred than those near the 3’ end of the nick which would only enter the F- bacterium if the entire chromosome is transferred, which happens only rarely.

Question

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
2
Correct. When performing a single interrupted mating experiment, all of the Hfr bacteria must be from the same strain, and all of the mating must occur at approximately the same time in order to ensure that the same donor genes are moving into the recipient bacteria at approximately the same rate. The remaining Hfr bacteria must be eliminated through selection. The order of traits transferred to the F- bacterium can be deduced by comparing the phenotypes of different batches of bacteria where mating was interrupted at different time points.
You have one more chance to answer the question correctly. Try again.
Incorrect. When performing a single interrupted mating experiment, all of the Hfr bacteria must be from the same strain, and all of the mating must occur at approximately the same time in order to ensure that the same donor genes are moving into the recipient bacteria at approximately the same rate. The remaining Hfr bacteria must be eliminated through selection. The order of traits transferred to the F- bacterium can be deduced by comparing the phenotypes of different batches of bacteria where mating was interrupted at different time points.

Question

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
2
Correct. Interrupted mating experiments can only provide information about the donor, Hfr chromosome. Interrupted mating experiments provide no information about DNA sequence or genome size but can determine the gene order on the Hfr chromosome.
You have one more chance to answer the question correctly. Try again.
Incorrect. Interrupted mating experiments can only provide information about the donor, Hfr chromosome. Interrupted mating experiments provide no information about DNA sequence or genome size but can determine the gene order on the Hfr chromosome.

Question

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
2
Correct. Although the strs allele from the donor could recombine with the bacterial chromosome and convert it to streptomycin-sensitive, these recombinants will be eliminated during selection. For linear DNA, as the donor DNA in an Hfr conjugation is, there must be two crossover points for recombination with the bacterial chromosome. If streptomycin is used as a selection agent, the surviving recombinant bacteria must have had both crossover points after the str gene on the donor DNA.
You have one more chance to answer the question correctly. Try again.
Incorrect. Although the strs allele from the donor could recombine with the bacterial chromosome and convert it to streptomycin-sensitive, these recombinants will be eliminated during selection. For linear DNA, as the donor DNA in an Hfr conjugation is, there must be two crossover points for recombination with the bacterial chromosome. If streptomycin is used as a selection agent, the surviving recombinant bacteria must have had both crossover points after the str gene on the donor DNA.

Question

An Hfr bacterial strain with a genotype of amps, gal+, leu+, cys+, thr+ was used in an interrupted mating experiment with an F- bacterial strain with genotype ampr, gal-, leu-, cys-, thr-. At regular intervals, the mating was interrupted and bacteria were selected with ampicillin and tested for the transfer of Hfr alleles into the F- bacteria. The time required to transfer the donor genes is shown in the table below.

Gene transferred Time
cys 3 min
thr 6 min
gal 10 min
leu 12 min
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
2
Correct. Since there are four minutes between thr and gal and two minutes between galand leu, the distance between thr and gal is greater than the distance between gal and leu. Although most bacterial chromosomes are circular, this information can’t be obtained from the data given. The order of the genes on the Hfr bacterial chromosome is cys thr gal leu.
You have one more chance to answer the question correctly. Try again.
Incorrect. Since there are four minutes between thr and gal and two minutes between galand leu, the distance between thr and gal is greater than the distance between gal and leu. Although most bacterial chromosomes are circular, this information can’t be obtained from the data given. The order of the genes on the Hfr bacterial chromosome is cys thr gal leu.

Question

An Hfr bacterial strain with a genotype of amps, gal+, leu+, cys+, thr+ was used in an interrupted mating experiment with an F- bacterial strain with genotype ampr, gal-, leu-, cys-, thr-. At regular intervals, the mating was interrupted and bacteria were selected with ampicillin and tested for the transfer of Hfr alleles into the F- bacteria. The time required to transfer the donor genes is shown in the table below.

Gene transferred Time
cys 3 min
thr 6 min
gal 10 min
leu 12 min
tHUKkrzyXMsWoD0iatgO5FfenpW6aXzsSttsjqJF1cWXTDCFVjoYhkhXdr83eBYrxSnMImF/t5cjQjp0jlDQq14Zb0s0ytFoxsr8KVlsG5TZ4nNuXrG0MXlJ8M9D5WPkIc+kQwmQ7NgeG2H/0/IW99EEfvuDAZHwPkVT7valymyPKRO1dhuSseNwIsHF/qxv60Ha6soonKsXoC/Z2jpKEc+6lzZ0H8WBaWVaRgCzkBk7P/cd9yp12HhHu139+S9QIaszW/390+vieh257crtkBbkY+GRxfv5+86HLAQFGb7h7PeU
2
Correct. From the data given, the order of genes on the Hfr donor chromosome is cys thr gal leu, but leu gal thr cys is equally correct. Since most bacterial chromosomes are circular, one can deduce the order of genes, but the orientation of the genes depends on the orientation of the F factor integration site.
You have one more chance to answer the question correctly. Try again.
Incorrect. From the data given, the order of genes on the Hfr donor chromosome is cys thr gal leu, but leu gal thr cys is equally correct. Since most bacterial chromosomes are circular, one can deduce the order of genes, but the orientation of the genes depends on the orientation of the F factor integration site.

Question

An Hfr bacterial strain with a genotype of amps, gal+, leu+, cys+, thr+ was used in an interrupted mating experiment with an F- bacterial strain with genotype ampr, gal-, leu-, cys-, thr-. At regular intervals, the mating was interrupted and bacteria were selected with ampicillin and tested for the transfer of Hfr alleles into the F- bacteria. The time required to transfer the donor genes is shown in the table below.

Gene transferred Time
cys 3 min
thr 6 min
gal 10 min
leu 12 min
NUfIVs3zWIwKDcO10hu6PCAIhcU5H9yOgFaH7uQkZn+6zjR/6NcKpoPEVKs0pFiF8GD6r0mipgDnfqv08WVjojAD7ZQfWJE+SgxktDdaBag/jtm1FVSaQwFT4s+vxLN24bT14XJBPbUEMGa1ypu1siVi6Gd2J9HYeni3rGgnp6OqsmnXwkKTEmQZDgWLJDapXXseUlI9bJRlNS0SxmWtzOL4eKbVJp6CNm+E7NxASICQ35iIMa8y/kshgQUV6cKlY2Tam4X5pXigqapwp8r48nUSTS/61TNtBaN8578CBhZBRdvay/7LjpDT6MI=
2
Correct. Since the cys+ allele from the Hfr strain appears first, this gene is the closest to the F factor origin of transfer.
You have one more chance to answer the question correctly. Try again.
Incorrect. Since the cys+ allele from the Hfr strain appears first, this gene is the closest to the F factor origin of transfer.