plasmid_cloning

Question 1 of 8

Animation Assessment

Question

The vehicle that carries a piece of foreign DNA to be cloned into a bacterial cell is called:

E. coli.

a restriction enzyme.

an insert.

a fragment.

a vector.

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Question

A plasmid vector generally contains which of the following?

An antibiotic resistance gene

A cloning site

A replication origin

A and B

A, B, and C

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Question

Restriction enzymes are used during DNA cloning to:

allow for selective growth of bacteria.

give the bacterial cell antibiotic resistance.

destroy contaminant DNA.

produce sticky ends on the vector and target DNA.

insert a plasmid into a bacterial cell.

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Question

Which of the following is true of DNA cloning?

It is used to isolate specific sequences of DNA.

It requires the enzyme DNA ligase.

It produces hybridized bacterial cells.

A and B.

A and C.

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Question

A recombinant plasmid contains:

a foreign piece of DNA.

E. coli genes.

an origin of replication.

A and B.

A and C.

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Question

The process by which a plasmid vector is introduced into a bacterial cell is called:

DNA cloning.

transfection.

introduction.

colony infection.

transformation.

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Question

Why is ampicillin added to the agar medium upon which transformed bacterial cells are grown?

To destroy unreplicated plasmids

To select for cells containing the vector

As a necessary nutrient for bacterial cell replication

To produce “sticky ends” on the plasmid

To kill pathogenic bacterial cells

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Question

Many copies of a recombinant plasmid are produced by:

insertion into the host bacterial cells genome.

replication of the plasmid DNA using the host enzymes and cell division of the host cell.

the plasmid genome contains its own replication genes which make copies of the plasmid.

the host cell copies the plasmid and infects other bacterial cells with it.

After cloning there is only one copy of the plasmid.

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Chapter 19.1. Animation Activity: Plasmid Cloning

Animation Assessment

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