IMPORTANT TERMS

recombinant DNA technology

genetic engineering

biotechnology

restriction enzyme

restriction endonuclease

cohesive end

engineered nuclease

gel electrophoresis

probe

Southern blotting

Northern blotting

Western blotting

gene cloning

cloning vector

cosmid

bacterial artificial chromosome (BAC)

expression vector

polymerase chain reaction (PCR)

Taq polymerase

DNA library

genomic library

cDNA library

positional cloning

restriction fragment length polymorphism (RFLP)

DNA sequencing

dideoxyribonucleoside triphosphate (ddNTP)

next-generation sequencing technologies

DNA fingerprinting

microsatellite

short tandem repeat (STR)

forward genetics

reverse genetics

transgene

knockout mice

knock-in mice

direct-to-consumer genetic test

gene therapy