1.What is semiconservative replication?

2.How did Meselson and Stahl demonstrate that replication in E. coli takes place in a semiconservative manner?

3.Draw a molecule of DNA undergoing replication. On your drawing, identify (1) origin, (2) polarity (5′ and 3′ ends) of all template strands and newly synthesized strands, (3) leading and lagging strands, (4) Okazaki fragments, and (5) location of primers.

4.Draw a molecule of DNA undergoing eukaryotic linear replication. On your drawing, identify (1) origin, (2) polarity (5′ and 3′ ends) of all template and newly synthesized strands, (3) leading and lagging strands, (4) Okazaki fragments, and (5) location of primers.

5.What are three major requirements of replication?

6.What substrates are used in the DNA synthesis reaction?

7.List the different proteins and enzymes taking part in bacterial replication. Give the function of each in the replication process.

8.What similarities and differences exist in the enzymatic activities of DNA polymerases I and III? What is the function of each DNA polymerase in bacterial cells?

9.Why is primase required for replication?

10.Why is DNA gyrase necessary for replication?

11.What three mechanisms ensure the accuracy of replication in bacteria?

12.How does replication licensing ensure that DNA is replicated only once at each origin per eukaryotic cell cycle?

13.In what ways is eukaryotic replication similar to bacterial replication, and in what ways is it different?

14.What is the end-replication problem? Why, in the absence of telomerase, do the ends of linear chromosomes get progressively shorter each time the DNA is replicated?

15.Outline in words and pictures how telomeres at the ends of eukaryotic chromosomes are replicated.