KEY TERMS

proteome

assay

homogenate

differential centrifugation

salting out

dialysis

molecular exclusion chromatography

ion-exchange chromatography

affinity chromatography

high-pressure liquid chromatography (HPLC)

gel electrophoresis

isoelectric focusing

two-dimensional electrophoresis

gradient centrifugation

sedimentation coefficient (Svedberg units, S value)

antibody (immunoglobin)

antigen

antigenic determinant (epitope)

monoclonal antibody

immunoprecipitation

enzyme-linked immunosorbent assay (ELISA)

western blotting

Edman degradation

mass spectrometry

MALDI-TOF