PROBLEMS

Question 5.1

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Question 5.2

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

Question 5.3

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Question 5.4

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Question 5.5

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Question 5.6

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

Question 5.7

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Question 5.8

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Question 5.9

Protein-purification problem. Complete the below table. ✓ 4

Purification procedure

Total protein (mg)

Total activity (units)

Specific activity (units mg−1)

Purification level

Yield (%)

Crude extract

20,000

4,000,000

 

1

100

(NH4)2SO4 precipitation

   5000

3,000,000

 

 

 

DEAE–cellulose chromatography

   1500

1,000,000

 

 

 

Gel-filtration chromatography

     500

   750,000

 

 

 

Affinity chromatography

       45

   675,000

 

 

 

Note: DEAE (diethylaminoethyl) bears a positive charge, and so DEAE-cellulose chromatography is anion-exchange chromatography.

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Protein-purification problem. Complete the below table. ✓ 4

Question 5.10

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Question 5.11

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Question 5.12

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Question 5.13

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Question 5.14

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Question 5.15

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Question 5.16

I5TGhCK5VFvWKEILuPmfkR3hwUDxZhEqJ1E9Hf21b+JB2uBo/Y4Z74NYT7yWnvApm2cjVqKtiH0WFH+092dDGYx2fP34115pyF5pG6ZuUYPHQKsUaKn6HRk0Q2/yFn+YpjxnVVFTg4fU+83l6Sj/AQ==

94

Question 5.17

8/sWQGiPOHsI8TCKos+VwLOW+ShO7nnKZL5IPvteZ3k10yQnsHJ7WwY9PtklJvBiG9MELFJiJ09XMpYzyiLQvfZF7NU12e7lkQEn9QYVTq8eN6UYt18cu9whPWv0FCE/ukSkK5q3JPZWX/6oPEH7uxHW8/0heKop8wkHelS9dRnNNW09b8CnXKJyA0hbF3fs85dujDeb5CZRqcTaRfSdQrXhKBtqBoieg7bCvjSC2oZ2SrPEiQA7ffhoSyvPPDNKr0Xbnx2hhE9O4GgjUnZunnHuHuaH3o4XkeY0ioZukDJdUkg29qHC0zwf+X9HF+bMXsIHf4p413IwX9S29/sNztMpeps=

Question 5.18

03CohrSgYUQEFi4CFMjPZP4alTBXQIqBM+ItEWEXn2VX5RepUx0ZZSnhSN7M7pn2WN2LwM7PweAPxS04OiIm+D/JLLBRhj6GMnkSxeztzP9k/sTZbozlnd4C3SQTmTbrUi8pTvYJBPtTTEuqmejhrQ+ORHe1RNfTMRs1TBsEyr5kAkLcECgadQIdgN6NVp/yWMNC5uaJuYOO3prqXC1GvmKh+sLVZSxuJPrvxcJ4FQeSTTjgQ5N5ZW3e+NMYk+VbRnA3tn0czKILt6zwqNsYmSqnU6++fVJ81y4Ad5Sl83cXqHUsdynxBEnqn59K8klKMKvkZsKUePRfVG+4kBHhMXJy4ZyEMDF1UG2D2II2z7rAsIOaTMI7t9NIgYI2NEL4whKErDXUja92fzCgJ/LgxkLCdjKvjIIJ4vIJYJqlfRwbrU4ODIyJgtcL68w=

Chapter Integration Problem

Question 5.19

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

Data Interpretation Problems

Question 5.20

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Question 5.21

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

Question 5.22

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Challenge Problems

Question 5.23

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

Question 5.24

VyHXdbg3/P1y9xsTgl1OBfiZ2nD91pV/DSVDMdBo88L8n/2iX+wQXD+rIotQVFy/RJ+G4uCI176rfUd0zTOWup7uaL2JD+9z+W068PBVKltfdbzcN7IGSGl9YcxI+2S+UigQzbkIzY64HI4pFIVecbwPm66NMtz/sHZKEuCCoNEFrb2SlFc1luP2vW5/3KfNFhdQYqArl0r3bTVqShr9j74s1N+zbvqhC8V0+ZfTHuQxLMJsEm24SXwubOLd5iK+Uyi4t8moH0uwv9EasGuwStNaZcYV/hm++6V1Rdl1yMXR9YUrVZF7HDbojM+A1vzJdukaSsUqupdnafzv8F9yvLJ9EID/zYvhwMlxGmbxMGIckuWKCVbcQxeHr1HYxYvNzWFLFpKoKqz4I0XqKFYA3+DSp60c1WN6

Selected Readings for this chapter can be found online at www.whfreeman.com/tymoczko3e.