Question
5.1
jNPVP1yJfoYaPqWt8qNKDCvHHqz36YLA4WrVg7moBD7EdRCJQ5YrfnWeC3m6QLOt0ZFkV0525R9/cHegAOhfFJSUNBZ/dHmzelLStpmiz0ApXt3PdPkdUcNvnOx3FvQ3RqmiigEHCkYBVaPiZrbIGVy+q13gTBdeDvizTLdhznLh7ydPw/r9Zg==
Question
5.2
LmQudV4zM+HUqay6Vb9H0MN1nc3baDTstkOlucUYhuy5E0+cH7WZvFtmu1KM0ggVJ1RfZTY9VKiJaIzZyTcbjv2r94KXajL67OiqBtU3nGmN6D0hHO/ei7NgTcKP9Alq/AxwhFZPgAlvWGSKRzC9hzbNMNnfGA2+csSleZ+aRaEsDj46wVyvrkrr3MjjKAX2Efj2+e/1/1BkeiFhIwx6q8N0LUi7bH+m3Hl+fd+b9+WSN7YGzq/lohjwf1lGcR4C0OSxEnAjNXry9Mw2ZMfjkSPGR0LJ8VwpalDwEeob4uVLKeNw58w3L3FeMt1xqJXj/ILaFekHBetV6pJ0Hqe6AmWzVxJooKxsSNlIpVWWPNv5p+fFB6X3/eH/R3DQK0/EpPAPkdHB1stShbYUkq+VD4cgveYjgOWYSjOkdwqc8RJkgBGbMfqCdz2XeDN3abJObmovk+AWT0568HY8262eoszpLwPfHzfmkAHwBV7dn8oxzKweew+WXirrYvElN5S2dvzY/py2nHf6NSelptVlaOYdHjMLHDAI/CMQmwQilOP6LVtBEgrnYHSGhHJOD9+U2Y3iYPMjH5OcWHykBZGSYnU1QSyjmhaOQZ2UfdutsGLYYJokPv3XdByIQHeZpKknx4rvque3j8EZanjGEIshTZ1jUQhBrl4sfozEzzj1zgB6wQbysz8mah45HmitQFUgkksiFx9dnc0qchaFwbZTGT0k6f5KVpvD1aCNo73o0K1GOXSNywPlqPHrkkCicWyJUyxCdhB3ZMVMhPxZr77xFvo+Kt6tjv/aEHbd0s27ZFlhPuHpkh4ipYGfbZ5QvIF5R9ycBJ3HUpdtaPQrHS5USx6D1bHSxw9oi7nKvw9mHLbwWu9EMUc7mTCpkz9as56gkhFyUAqtgCGXucvMnGwRZl6TSQID7u56iujYwxLZEGeK6Vebz7sJ0UVCAK6ouhJJnrNrRvy+t5qVLZFRAi14jFqtCuIsr1mbUFu9aZWfFmNlPZ2jqRmvLloQGcXkH6tPbHg0mpAjwF9Ysz3tLsVMqmZ5lEQd5VVyWeJwZ+cpYHHyW5zsaMW5s9Nvy2B/Rek2PDL3PTd6y8z87cnGJmsaaVLwjw7UXCIUxAIeNOhY4XD+Kz2hN5qRW4QkVJJCNx3Mz630Xh39UgiiUman2RuWZ54nD4edTanuDgx/IbN9kpnWJBTKZQOOJqPl96Vb0GEiGTa1QXHnmuczgBY7hQzq/Lus5Dfy7XQS5rdR4ukJgLUF6BpbnLyUK2qtoUJCGQr4yB2NZTNVFEaDvrfDryim9TCph4rpM2bAD8lXggchh9Y4tHKKygtxy0i58Qt7jtOgwltlj5rtkDYMtoL6dh47HR77niGHV6mB+v053g==
Question
5.3
NlrQ4DxTgO7KF5yg0jLnRrXjbYl0ZW+njEhsYvw6WhIJot1hcAYvNAXjEWWU4BLIz8QDrBLPKiCgjqRiGabQBvNzFe+5QgM+476cIgtnPiCuSmgygWwSublbmwk5gOsQ7kpg+fuHTo4Qy52ZeVoKaeo9ZiymzysqWRZWGT24HrcoQnYZ
Question
5.4
gZFwQHS9OZE8WAIwwvUv4mliUN2quBXTA31iwJ/MZ8a91nnU5LJ8uElnPwo1jh4ZNWF03zqpZVPMmPloN0Tr4jZZzi8ySNd8H6k0pRq9WWgzyucNaRfnoZDK1MEB9AbaDLeT7CHF//a8X9iR0Uxl5Rbtt1GBKXIaPUhijjf00G53aLFLgOa269mFd9Z0Od1nCY74p3lyq7IIHntII5JmiVTtpvwCTeF2hVjhrIHVnjZTmCou5LaLkwyJkJguzb9u
Question
5.5
pT+qibrJVUZXGaNRr2Sz0NmG0ldCEmnqZM8H8j/36dNSU10AgoyAIWWqY5d/lOhYyvve+Q6RB/QAkL6OBfCADPCipccNtdviNus5HmOfnBWXZLdm7lIQ/3Nf2PeE+WS3NT/PekNauBodyb3p14RM2Q==
Question
5.6
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
Question
5.7
ZVcGkdDXNlI4+UD4khuFhaVMBUhl4XAO1es4ZSLF6Wn6JN1zWbpPIYhK8G+hK6TPHLxW4WD64/W4MeBdD/c3ZfQJ9+7LPrI7PPvxdRzsh2pw4nIIKJUSkhfSLS7fSrLguXy1IJgzMgIIlPfdC95eR2l0oE5ZI6syZP8BbCGJVYZols3sWnsnZ1S45oTb5SFWOWPijg==
Question
5.8
dEnRJt3IIpWIhTmdPzBqnWCWCVOqgiSxX88UeQPFPB0DKu7CQiy3Z04EQu7wq7AapIip0lGEn8SMowKnxskkW12a7yFzKBuk1LjmUWMZ9wkWZ18iHyOe+DAvEK0mvTYTYQuEw9v1veznZtmr2cJPuZJz297RNz3Adwu08w7XqPV3yTQLwpkvYZEv+EszkpP0B9U88ADEmgbbGQD1YRicNCnEfqG4TgaR8xp+ORPOi9iQJxNukKzQScrqaPgw7F6/xG8iKQe931TxZ8LHMFXuC0Rw0kE7RcT6E23pRNoeCKCNsamhWI33NT0xK506MNUwfHiIXP407ZchkM6qEtLvW6zuV0JaH242qkRZd0+g/CG7Wf918SH/CMEaaDZWfI7lT6OOhOsFEIOG8ZQwxmUd8OH5wWycNRr+NvknT/kflieyEf9KM19mwUhy0909GsdbnLcVWS/t5kI=
Question
5.9
Protein-purification problem. Complete the below table. ✓ 4
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Specific activity (units mg−1)
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DEAE–cellulose chromatography
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Gel-filtration chromatography
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Note: DEAE (diethylaminoethyl) bears a positive charge, and so DEAE-cellulose chromatography is anion-exchange chromatography.
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46O/GvlXlao=
Protein-purification problem. Complete the below table. ✓ 4
Question
5.10
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
Question
5.11
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
Question
5.12
8FaoDysE1h3iMX3znxspqy2Tq6FLmr0JwJ6a4QK7284kPAVnnHWloomKhqpWnyV8iFTySoSjmIGrt0nfjksZO5/+zw/0cG5DKXXPZE/S+HGISRnAEv71TFLLhtw/7m/7QmZYzH6G61Phe/aWyBm7MqW+CtVZqYb2oe9+/jDzXy92rE2G0Zf7e04uMytMx87id9QdRt2ofH9STg6SMnHaJZ9exDQyLB3gDuxmRv/SxRY=
Question
5.13
6doshhjNdryVEX0BiXiQLHZ+wAv10v5q/PR4Spx4Xx1Wzpec6PVAQZHc/ytdkvi0tSu4BCZQ9tD/Nn4xNfAaAfjURCAjbz6deufqs6VSdqkOH/QBetbzhD8cUfidsmMN3wm8SbthlAxdhv6W3qkBvLArJJm6TcXlSFke5jsMv8IBDPL7
Question
5.14
7O+s/awPYqZm/H0CcUWsFk//ETTn7DJBxFbeCRpJhUcnzrzDYL1itMq0iB8i0qeefW1q52RVOIBsSR0nA0NIqCmRMRym/D2uWz6iHoa3xuJRKi+RJMed7vb0yeKrw9+RSpl8gdwVSA6Ycg2L81m79HOnq8ucpu/PwjvPp/NiGOj715RHPv/+R7C1dVU=
Question
5.15
CnvXL5glMa2JH6PqpviQtMYaDYJsWxLe8JZPdUnOzYqMwhWtqe3vqYgREeNJfEDFY18UM9HMBq3aoS4YUMaxLPHtR3m7bRpW+J+/+AnCz5vDXxS+rOxGU71bM4ZIOJHHthnEk0Ye4Y53dGRY3xoMee6D0qEj/7DJ
Question
5.16
I5TGhCK5VFvWKEILuPmfkR3hwUDxZhEqJ1E9Hf21b+JB2uBo/Y4Z74NYT7yWnvApm2cjVqKtiH0WFH+092dDGYx2fP34115pyF5pG6ZuUYPHQKsUaKn6HRk0Q2/yFn+YpjxnVVFTg4fU+83l6Sj/AQ==
Question
5.17
8/sWQGiPOHsI8TCKos+VwLOW+ShO7nnKZL5IPvteZ3k10yQnsHJ7WwY9PtklJvBiG9MELFJiJ09XMpYzyiLQvfZF7NU12e7lkQEn9QYVTq8eN6UYt18cu9whPWv0FCE/ukSkK5q3JPZWX/6oPEH7uxHW8/0heKop8wkHelS9dRnNNW09b8CnXKJyA0hbF3fs85dujDeb5CZRqcTaRfSdQrXhKBtqBoieg7bCvjSC2oZ2SrPEiQA7ffhoSyvPPDNKr0Xbnx2hhE9O4GgjUnZunnHuHuaH3o4XkeY0ioZukDJdUkg29qHC0zwf+X9HF+bMXsIHf4p413IwX9S29/sNztMpeps=
Question
5.18
03CohrSgYUQEFi4CFMjPZP4alTBXQIqBM+ItEWEXn2VX5RepUx0ZZSnhSN7M7pn2WN2LwM7PweAPxS04OiIm+D/JLLBRhj6GMnkSxeztzP9k/sTZbozlnd4C3SQTmTbrUi8pTvYJBPtTTEuqmejhrQ+ORHe1RNfTMRs1TBsEyr5kAkLcECgadQIdgN6NVp/yWMNC5uaJuYOO3prqXC1GvmKh+sLVZSxuJPrvxcJ4FQeSTTjgQ5N5ZW3e+NMYk+VbRnA3tn0czKILt6zwqNsYmSqnU6++fVJ81y4Ad5Sl83cXqHUsdynxBEnqn59K8klKMKvkZsKUePRfVG+4kBHhMXJy4ZyEMDF1UG2D2II2z7rAsIOaTMI7t9NIgYI2NEL4whKErDXUja92fzCgJ/LgxkLCdjKvjIIJ4vIJYJqlfRwbrU4ODIyJgtcL68w=