PROBLEMS

Question 8.1

sgWJ5bPWmSqu8HQ6VntkH7PKz/vCo76UZrzBYMyJqbZBxH90GnelxynzTNcPodxK9VJCJKMsHi5FtD4HJCkooKJevsBaNUSP2oxAR8NEU2JasaxupY0oEairJ1EWzIYOKKlQ2w==

Question 8.2

ipnk/955Bs4CgjqyoEdst2tS1tZzFAnTxUmZ39VKHVgYWNa0e6eYHtOWdM2u4xGW/nkFoJOK7kBguH7OP5dX7d5vdhDFM8/qjkeNUKNagDIsl6fyvUntOjVCPHPfL8lz1+PNk0lRh5fIiNcdUsyquxfn3dybjqx1YaevK94QrQANkOvFLZuk7xg9CNpl89U9Ci6mkrbB6cAh7vI9HEKk2QiiTCB7RXIEcbRJwaIZM985H+lcuBV+/amjCCP0hCHTrrD7ubGI7XNCI8kWApgVUxx/hup+RLHNx5rJGA0wtdzlFCwuyfmgOhI0S7PDEC5W

Question 8.3

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

Question 8.4

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Question 8.5

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Question 8.6

Mode of inhibition. The kinetics of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2 μM inhibitor. ✓ 6

 

Velocity (μmol minute−1)

[S] (μM)

No inhibitor

Inhibitor

  3

10.4

  4.1

  5

14.5

  6.4

10

22.5

11.3

30

33.8

22.6

90

40.5

33.8

(a) What are the values of Vmax and KM in the absence of inhibitor? In its presence?

(b) What type of inhibition is it?

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Mode of inhibition. The kinetics of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2 μ M inhibitor. ✓ 6 (a) What are the values of V max and K M in the absence of inhibitor? In its presence? (b) What type of inhibition is it?

Question 8.7

A different mode. The kinetics of the enzyme considered in problem 6 are measured in the presence of a different inhibitor. The concentration of this inhibitor is 100 μM. ✓ 6

 

Velocity (μmol minute−1)

[S] (μM)

No inhibitor

Inhibitor

  3

10.4

2.1

  5

14.5

2.9

10

22.5

4.5

30

33.8

6.8

90

40.5

8.1

(a) What are the values of Vmax and KM in the presence of this inhibitor? Compare them with those obtained in problem 6.

(b) What type of inhibition is it?

46O/GvlXlao=
A different mode. The kinetics of the enzyme considered in problem 6 are measured in the presence of a different inhibitor. The concentration of this inhibitor is 100 μ M. ✓ 6 (a) What are the values of V max and K M in the presence of this inhibitor? Compare them with those obtained in problem 6. (b) What type of inhibition is it?

Question 8.8

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Question 8.9

Y4QE84/wFI91Wp2h7QfOYB2pMoojWJX1RYGBR5WcxSsk/gyK8/KqMYp9XIN0OUhERryV3wFMS5/5d8V9gtbXLU79OMEnCAyWItleSvXuNVoe0iT425D6AwHXwVNhOp8h0yT9KpY3+QR8mNd1IHFNWba1dwQLD6bTihyqpX+1Lf+Ug5+voIle++2XyG31zC7cOVOuW44QV7itH6pyat0WLCEVbJWxsB48vjmZ/erfuB38hDeOw48+VLWqVYmLgUsTn7T1L20tvB7yy93JY367wOQTFcVCnVbdAwbfw1A5u7sumrbRe1a5bat9XGqVShoJklvP8zkGXHWa7r7GeGFI+jGQMTEGijF7Js2VQmB1OG0I4DuHgNJph4q38var3QT3/71frH2n1aDBOV+yXgzmOMlDOtcXH5gb

Question 8.10

GM4p2SUQp1OQYlzkx4boeOuRxwt1Scn39US9rxQpzNUW9s0djMN2ommCDcT8DAZqw71plgl3HLFC53vswzFEja73d//pRE1nzawe5i+OrtWQrLfyg0stKhUHBuBxmnNEaxoLvkkMvK4v/t0UnPNcNUcTPvK5MtosVUuidC8wyZSn8krlO0lgj7YQ/oRkVDAQGdbnhA==

Question 8.11

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Question 8.12

Btc1u8c49mMSsl+GMjKvQqQaqTGF47161IQYNt/qVN2HiZUg2lBIVQATCUcmWoHpOp87GQ+gqIeYfuNr0wE6Sbfv+zJO3t37oTstCAuetZ/Tkc0u7Xe9OyLT+2nB4j9PdUnEWw+/jiYp9VDkR9dbs/Rqv9+avupKGerFYE8a8UlO35fiRhwzJDg5XypGRgKhtoOgBEI6L321SZOmqVmIwcFeLlhb1FLh6ymPbQv6fal77v1mqM241wPOyd4Cu4MOzKXLtJ2dOhaXLjD549j2Gk89x+i1Rbl0Yx/miX5K35IKjWHSW9zUmXexF4PCKt4b

Question 8.13

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Chapter Integration Problems

Question 8.14

Mental experiment. Picture in your mind the velocity-versus-substrate concentration curve for a typical Michaelis–Menten enzyme. Now, imagine that the experimental conditions are altered as described below. For each of the conditions described, fill in the table indicating precisely (when possible) the effect on Vmax and KM on the imagined Michaelis–Menten enzyme.

Experimental condition

Vmax  

KM

(a) Twice as much enzyme is used.

(b) Half as much enzyme is used.

(c) A competitive inhibitor is present.

(d) An uncompetitive inhibitor is present.

(e) A pure noncompetitive inhibitor is present.

 
46O/GvlXlao=
Mental experiment. Picture in your mind the velocity-versus-substrate concentration curve for a typical Michaelis–Menten enzyme. Now, imagine that the experimental conditions are altered as described below. For each of the conditions described, fill in the table indicating precisely (when possible) the effect on V max and K M on the imagined Michaelis–Menten enzyme.

Question 8.15

e6pzuYF2AqehCOrxeVLn+zNwFekciTrxgMIRfRskaBmvzWJaAywR+KIauhFuYhm07MuQuzkf04Qz2yaeWI85h7ws7QuX17JhVl8o/ddtcnJyL1zSfa8mccKp5saZHc/8QTkKelvnsSpnnM3jz1W2Hjcw1c2H+RvkClqps+pg7clgpd1PTHCmXLLapbF4XHg41/gMYA4JAYdCR/aQntflNKxVHfYjmIsAZlzV6A==

Challenge Problems

Question 8.16

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Question 8.17

HnWcwPNZQg/vU/tBMZSpea5U+U9wT1FrbZByotYaCeKgD65ZazbPGvSdsL2nW7oB/T5lFciwTQIQNDKnl6riFOsKouNVnuGjTjzhruTaWrI0GXnS/aSR1sFUuoGs8DDXQUNwCeD7zvd/+PPEb3AC4i05xo8bFTHW

Question 8.18

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

Question 8.19

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

Selected Readings for this chapter can be found online at www.whfreeman.com/tymoczko3e.

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