A sentence should flow from subject to verb to object without unnecessary pauses.
The comma should not separate the subject, zoos, from the verb, are becoming.
The comma should not separate the verb, explained, from its object, the subordinate clause that she was busy and would see him later.
EXCEPTION:Commas may appear between a verb and its subject or object only if another sentence element requires them.
The commas separating the subject, National Zoo, from its verb, has, are required around the adjective clause which is on Connecticut Avenue.
Exercises:
Misuses of the comma 1
Misuses of the comma 2
Related topic:
Transitive verbs, objects, and complements