KEY TERMS

proteome

assay

specific activity

homogenate

salting out

dialysis

gel-filtration chromatography

ion-exchange chromatography

cation exchange

anion exchange

affinity chromatography

high-performance liquid chromatography (HPLC)

gel electrophoresis

isoelectric point

isoelectric focusing

two-dimensional electrophoresis

sedimentation coefficient (Svedberg unit, S)

antibody

antigen

antigenic determinant (epitope)

polyclonal antibody

monoclonal antibody

enzyme-linked immunosorbent assay (ELISA)

western blotting

fluorescence microscopy

green fluorescent protein (GFP)

matrix-assisted laser desorption/ionization (MALDI)

electrospray ionization (ESI)

time-of-flight (TOF) mass analyzer

Edman degradation

phenyl isothiocyanate

tandem mass spectrometry

overlap peptide

peptide mass fingerprinting

solid-phase method

x-ray crystallography

Fourier transform

electron-density map

nuclear magnetic resonance (NMR) spectroscopy

chemical shift