Chapter 3. 3.3 Immunoblotting

Introduction

Assessments for Animated Techniques
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3.3 Immunoblotting

Question 3.1

Protein immunoblotting is also referred to as KBBWrzfLptIa2utP blotting.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.2

A EGIMzzV7cEv+z1/o antibody recognizes a target of interest directly, whereas a rbe/2ARJ3Zsq6PH3zlww4A== antibody is designed to bind to the conserved region (Fc domain) of another antibody and is often conjugated to an enzyme or other molecular marker.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.3

The 6+VUN2sXfnKjiecg step is required to bind nonspecific sites of the nitrocellulose membrane so that the primary and secondary antibodies will only bind specifically to their target molecules on the blot.

_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.4

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.5

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.6

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_feedback_correct: Correct. _feedback_incorrect: Incorrect.

Question 3.7

Click on the step and the associated process to identify the correct sequence of steps that are required to set up the transfer step of the immunoblotting process, once you have run out your SDS-PAGE gel. Note: In the diagram, the positive side of the apparatus is where you will assemble your transfer experiment.

Immunoblot with Power Supply
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Question 3.8

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