EF-Tu–mediated binding of aminoacyl-tRNA to the ribosome. In the first step of elongation, the incoming aminoacyl-tRNA is bound by EF-Tu–GTP and inserted into the A site (EF-Tu is shown simply as Tu). GTP hydrolysis releases EF-Tu–GDP, leaving the tRNA in place. Through accommodation, the tRNA base-pairs with the mRNA codon and shifts by 70 Å into the correct position for the peptidyl transferase reaction. EF-Ts (shown as Ts) recycles EF-Tu by regenerating the EF-Tu–GTP complex.