Methods for visualizing gene expression in developing animals
The two principal technologies for visualizing where a gene is transcribed or where the protein that it encodes is expressed are (left) in situ hybridization of complementary RNA probe to mRNA and (right) immunolocalization of protein expression. The procedures for each method are outlined. Expression patterns may be visualized as the product of an enzymatic reaction or of a chromogenic substrate or with fluorescently labeled compounds.