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Figure 9.9 An Experiment Demonstrates the Chemiosmotic Mechanism

Original Paper: Jagendorf, A. T. and E. Uribe. 1966. ATP formation caused by acid-base transition of spinach chloroplasts. Proceedings of the National Academy of Sciences USA 55: 170–177.

The chemiosmosis hypothesis was a bold departure from the conventional scientific thinking of the time. It required an intact compartment enclosed by a membrane. Could a proton gradient drive the synthesis of ATP? The first experiments to answer this question used chloroplasts, plant organelles that use the same mechanism as mitochondria to synthesize ATP.