Microscopes reveal the features of cells

Most cells are too small to be seen with the unaided eye. Microscopes allow cells and details within them to be seen. The property that allows detail to be seen is called resolution. Formally defined, resolution is the minimum distance two objects can be apart and still be seen as two objects. Resolution for the human eye is about 0.2 mm (200 µm). Most cells are much smaller than 200 µm and thus are invisible to the human eye. Microscopes magnify and increase resolution so that cells and their internal structures can be seen clearly (Figure 5.3).

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Figure 5.3 Looking at Cells The first six images below show some techniques used in light microscopy. The last three images were created using electron microscopes. All of these images are of a particular type of cultured cell known as HeLa cells.

Note that the images in most cases are flat, two-dimensional views. As you look at images of cells, keep in mind that they are three-dimensional structures.

Activity 5.2 Know Your Techniques

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There are two basic types of microscopes—light microscopes and electron microscopes—that use different forms of radiation (see Figure 5.3). While the resolution is better in electron microscopy, only dead cells are visualized because they must be prepared in a vacuum. Light microscopes, by contrast, can be used to visualize living cells (for example, by phase-contrast microscopy; see Figure 5.3).

Before we look at cell structure, it is useful to consider the many uses of microscopy. Here is an example: an entire branch of medicine, pathology, makes use of many different methods of microscopy to aid in the analysis of cells and the diagnosis of diseases. For instance, a surgeon might remove from a body some tissue suspected of being cancerous. The pathologist might:

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