Phytochromes mediate the effects of red and far-red light

Photomorphogenesis refers to a number of physiological and developmental events in plants that are controlled by light. For example:

Action spectra of the above processes show that they are induced by red light (650–680 nm). This indicates that plants must have a photoreceptor pigment that absorbs red light and initiates photomorphogenesis.

What is especially remarkable about these red light responses is that they are reversible by far-red light (710–740 nm). For example, if lettuce seeds are exposed to brief, alternating periods of red and far-red light in close succession, they respond only to the final exposure. If it is red, they germinate; if it is far-red, they remain dormant (Figure 36.12). This reversibility of the effects of red and far-red light regulates many other aspects of plant development, including flowering and seedling growth.

experiment

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Figure 36.12A Sensitivity of Seeds to Red and Far-Red Light

Original Paper: Borthwick, H. A., S. B. Hendricks, M. W. Parker, E. H. Toole and V. K. Toole. 1952. A reversible photoreaction controlling seed germination. Proceedings of the National Academy of Sciences USA 38: 662–666.

Lettuce seeds will germinate if exposed to a brief period of light. An action spectrum indicated that red light was most effective in promoting germination, but far-red light would reverse the stimulation if presented right after the red light flash. Harry Borthwick and his colleagues asked what would be the effect of repeated alternating flashes of red and far-red light. In each case, the final exposure determined the germination response. This observation led to the conclusion that a single, photoreversible molecule was involved. That molecule turned out to be phytochrome.

work with the data

Figure 36.12B Sensitivity of Seeds to Red and Far-Red Light

Original Paper: Borthwick, H. A. et al. 1952.

Harry Borthwick, Sterling Hendricks, and colleagues at the U.S. Department of Agriculture performed a series of landmark experiments that suggested the existence of a receptor for red light that determined seed germination. Borthwick was the son of a leading plant pathologist who followed in his father’s footsteps and studied plant physiology. After it was discovered that plants responded to the length of day (photoperiodism; see Chapter 37), the importance of nonphotosynthetic responses to light as a developmental signal became a hot topic of research. For over a century, it was known that lettuce seeds required light to germinate. By placing lettuce seeds in an environment where other environmental variables such as temperature were controlled, Borthwick’s group tested the light signaling on germination.

QUESTIONS

Question 1

An action spectrum (see Figure 10.4) for seed germination was obtained by first soaking the seeds on wet filter paper in the dark for 16 hours for imbibition, and then exposing them to different wavelengths of light for 1 minute. Having been exposed to a possible germination signal, the seeds were returned to the dark and tested for germination after 2 days. The results are shown in Table A in terms of energy efficiency (how much light energy was required for 50% seed germination).

  1. Explain the energy efficiency measurement: what do high and low numbers mean?

  2. Plot the data as energy efficiency versus wavelength. What can you conclude about the most efficient wavelengths of light for germination?

Table A
Wavelength (nm) Energy required for
50% seed germination
560 35
570 25
580 15
590 10
600 8
620 6
640 4
660 3
680 4
690 45
700 80

A high number means that more light energy at that wavelength is needed for the response (see germination) than a low number.

The highest efficiency was in the red part of the visible spectrum (600–680 nm).

Question 2

There was evidence from other light signaling responses in plants that the light effects were reversible by exposure to different wavelengths of light. After the data in Table A were obtained, Borthwick’s team decided to first expose groups of 200 seeds to light at 660 nm (red, R) for 1 minute, and then some batches to light at 700 nm (far-red, FR) for 4 minutes. The seeds were returned to the dark and germination evaluated after 2 days. Table B shows the results.

  1. What can you conclude about the nature of the light signal responder?

  2. Why was there some germination in the “None” condition?

Table B
Wavelengths used Percent germination
None 8.5
R 98
FR 54
R then FR then R 100
R then FR then R then FR 43
R then FR then R then FR then R 99

The photoreceptor has two forms that reversibly interconvert: red light stimulates the formation of the active receptor, and far-red light stimulates the conversion of the active receptor to its inactive form.
A small amount of phytochrome was in the P-FR state in the dark.

A similar work with the data exercise may be assigned in LaunchPad.

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The basis for the effects of red and far-red light resides in the photoreceptor pigment protein in the cytoplasm of plants called phytochrome. Phytochrome exists in two interconvertible “isoforms,” or states. The molecule undergoes a conformational change upon absorbing light at particular wavelengths. The default or “ground” state, which absorbs principally red light, is called Pr. When Pr absorbs a photon of red light it is converted into Pfr. Pfr is the active form of phytochrome—the form that triggers important biological processes in various plants.

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The part of phytochrome that absorbs red and far-red light is a covalently attached pigment called a chromophore (Figure 36.13A). The chromophore of Pr preferentially absorbs red light; when it does so, it changes conformation and the phytochrome is converted to the Pfr form. When the chromophore of Pfr absorbs far-red light, the phytochrome is converted back to the Pr form. If you know organic chemistry, this reaction is a familiar cis-trans isomerization.

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Figure 36.13 Phytochrome Exists in Two Forms Absorption spectra of phytochrome reveal two interconvertible forms, corresponding to the cis and trans isomers of phytochrome’s chromophore. The Pr form absorbs red light; the Pfr form absorbs far-red light.

The absorption spectra for Pr and Pfr correlate with their action spectra (Figure 36.13B). As you have seen, phytochrome affects seed germination, shoot development after etiolation, and flowering. In Arabidopsis there is a gene family that encodes five slightly different phytochromes, each functioning in different photomorphogenic responses.

For a plant in nature, the ratio of red to far-red light determines whether a phytochrome-mediated response will occur:

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For some plants growing in the shade, the low ratio stimulates cell elongation and the plants grow upward toward the sun. The shade cast by other plants also prevents germination of seeds that require red light to germinate. The reflective properties of the soil can also affect the red to far-red ratio—and thus plant behavior. For example, cotton seedlings grow more slowly on soils (such as clay) that reflect more red than far-red light.