recombinant DNA technology
genetic engineering
biotechnology
restriction enzyme
restriction endonuclease
cohesive end
engineered nuclease
gel electrophoresis
probe
Southern blotting
Northern blotting
Western blotting
gene cloning
cloning vector
linker
cosmid
bacterial artificial chromosome (BAC)
573
expression vector
yeast artificial chromosome (YAC)
Ti plasmid
polymerase chain reaction (PCR)
Taq polymerase
reverse-transcription PCR
real-time PCR
DNA library
genomic library
cDNA (complementary DNA) library
in situ hybridization
positional cloning
chromosome walking
restriction fragment length polymorphism (RFLP)
DNA sequencing
dideoxyribonucleoside triphosphate (ddNTP)
next-generation sequencing technologies
DNA fingerprinting
microsatellite
short tandem repeat (STR)
forward genetics
reverse genetics
site-directed mutagenesis
oligonucleotide-directed mutagenesis
transgene
knockout mice
knock-in mice
gene therapy