TABLE 14.1 Characteristics of some common restriction enzymes used in recombinant DNA technology
Enzyme Microorganism from
which enzyme
is produced		 Recognition sequence Type of fragment end produced
BamHI Bacillus amyloliquefaciens image Cohesive
CofI Clostridium formicoaceticum image Cohesive
EcoRI Escherichia coli image Cohesive
EcoRII Escherichia coli image Cohesive
HaeIII Haemophilus aegyptius image Blunt
HindIII Haemophilus influenzae image Cohesive
PvuII Proteus vulgaris image Blunt

Note: The first three letters of the abbreviation for each restriction enzyme refer to the bacterial species from which the enzyme was isolated (e.g., Eco refers to E. coli). A fourth letter may refer to the strain of bacteria from which the enzyme was isolated (the “R” in EcoRI indicates that this enzyme was isolated from the RY13 strain of E. coli). Roman numerals that follow the letters identify different enzymes from the same species.