Chapter 1. “A likely story” - Enzyme Inhibition Case Study

1.1 Introduction

Lehninger Principles of Biochemistry
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A Likely Story

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Instructor Notes for “A Likely Story”, Enzyme Inhibition Case Study

Case Overview

Students will be given enzyme kinetic data; they will use Microsoft EXCEL (or another graphing program) to graph the data. They will then determine kinetic parameters and type of inhibition and correlate this information to descriptions of molecular mechanisms. The online assignment is made for students to work through individually or in groups and to answer the questions individually online, referring to their text. All assessments are automatically scored and a tutorial to guide students through using MS EXCEL is provided. Note: this case has two versions which the instructor can choose to assign that differ only by the inclusion of a question in which students are asked to calculate kcat from experimental data. Instructors should review this question and assign only one version of the case to students.

Topic Pre-requisites: Students should have exposure to the topics of sections 6.1-6.3 in Lehninger 6E. In particular, it is important to discuss mechanisms of reversible inhibition, irreversible inhibition, Lineweaver-Burk plots, and how to calculate kcat (if optional kcat question is assigned).

Learning Objectives

This case is intended for remediating or extending student capabilities in these difficult topics:

1) Real-world applications of the study of enzyme kinetics. Students should be able to:

  • describe the distinctions between kinetic mechanisms of enzyme inhibition
  • use inhibition kinetics to identify inhibitors by kinetic mechanism

2) Connections between molecular mechanism and enzyme kinetics. Students should be able to draw connections and distinctions between molecular binding mechanisms and kinetic mechanisms of inhibitors.

3) Practice critical thinking skills involving data. Students should be able to:

  • Evaluate data from tables
  • Create graphical representations of data
  • Calculate common kinetic parameters (Vmax, Km and kcat)from data

Questions designed to show areas of difficulty for students

1) Students often struggle with the practical calculation of kcat. This issue will be apparent if students incorrectly answer the optional question about kcat which may or may not be included depending on which version of the case chosen (see notes in Case Overview section above)

2) Students may mischaracterize the description of an irreversible inhibitor because they do not know what a stable Schiff-base linkage is. The case provides a teachable moment to point out that the student should learn to recognize when they do not know a term and remedy the problem.

3) Students often have difficulty identifying a noncompetitive inhibitor when using real data because real data is not ‘perfect’; fit-lines from real data are unlikely to intersect perfectly on the x-axis in a Lineweaver-Burk plot.

4) Similar to the issue above, students will often assign mixed inhibition to an uncompetitive inhibitor.

5) The example of a lab technician neglecting to properly label samples is, of course, a very common issue in teaching and academic research laboratories. This oversight was inserted in the case study as a teachable moment to remind students to be sure to properly label samples throughout their manipulations when they carry out their own laboratory work.

Suggested implementation

Below we describe two options for course implementation. The hybrid Online/In-class approach is recommended. Time required for students to complete the online case will vary greatly depending on students’ prior experience in using MS EXCEL or similar program. The case study can be started and stopped, and so it is recommended to give students a window of 2-3 days in which to complete the assignment.

Hybrid:Online/In-class : (recommended approach; ~30 minutes of class-time expected)

1) Share the case study link with your students to work online outside of class and assign it to be due before your next class meeting. Students should be instructed to bring copies of notes, graphs, and calculations to class.

2) Review the online answers before the following class for difficult areas for students (see expected areas of student difficulty above).

3) Lead students in a discussion in pairs, groups, or as a class (depending upon class size and instructor preference) to address unresolved difficulties (~30 minutes in-class time).

4) It’s recommended after using the case to select from the supplied assessment questions to use on exams or homework assignments to reinforce the difficult concepts covered. Please see the document “Exam Questions Case 1- Enzyme Inhibition”

Online only approach: (minimal in-class time required)

1) Share the case study link with your students to work online, preferably in pairs or groups of three. Assign the case study to be due before your next class meeting.

2) Review the online answers for difficult areas for students (see expected areas of difficulty above).

3) Mention or remediate tough points during a portion of lecture.

4) It’s recommended after using the case to select from the supplied assessment questions to use on exams or homework assignments to reinforce the difficult concepts covered. Please see the document “Exam Questions Case 1- Enzyme Inhibition”

Suggestions for in-class discussions (these questions may also be used in summative assessments, i.e. exams, scored quizzes, etc.:

  • What type of kinetic mechanism would be expected for an irreversible inhibitor and why?
  • Students could be directed to brainstorm in groups or as a whole class.
  • Have students read/view descriptions of inhibitors in the case: ask what specific information in each description is relevant and how it indicates the expected kinetic mechanism.
  • Could be easily used as a think-pair-share exercise.
  • How could an investigator distinguish between a noncompetitive inhibitor and an irreversible inhibitor?
  • Could be used when students have difficulty with questions 8 & 9 from the online activity.
  • Why does V max remain unchanged for a competitive inhibitor?
  • When calculating k cat , when should you use E total and when should you use [E total ] and why?
  • Could be used when students have difficulty with optional kcat question from the online activity.
  • Why does the measured K m value go down in the presence of an uncompetitive inhibitor?

You are missing vital information

You may be missing vital information needed to sufficiently explain this incident. You must complete all investigations before proceeding to the final assessment questions.

You are missing vital information

You may be missing vital information needed to sufficiently explain this incident. You must complete all investigations before proceeding to the final assessment questions.

This activity has already been completed, however feel free to review the information contained within.

You are about to leave the investigation and proceed to the final assessment. Are you sure you wish you to proceed?

Figure 1.1

“I’m telling you I didn’t do it!” cried Mrs. Bleu. “He just fell on my knife!” The detective smirked, turning away to hold back a chuckle.

“I don’t know why” she continued, “ he ju—, he… he just sort of turned to me gasping and fell forward... I was just making dinner, chopping broccoli...” Mrs. Bleu trailed off and looked longingly at the floor of the interview room. They had been there for hours. “He was just standing at the end of the kitchen drinking his coffee and looking through the mail when it happened, but it was an accident. Why would I kill my husband?”

“We don’t know why”, the detective said calmly, “we’re hoping you’ll tell us. Your husband was found on the kitchen floor in a pool of blood...his blood. He had a knife-wound, a wound from a kitchen knife that was lying on the floor just feet away covered in his blood. His blood…and your fingerprints! Moreover, you told the 911 operator that youstabbed him—“

“Accidentally!” she gasped.

Figure 1.2

“Ok…ok, you admitted accidentally stabbing him…but do you really expect us to believe that he just fell onto your knife. Do you know how ridiculous that sounds?”

“I don’t care if it sounds ridiculous! It’s what happened!” she said, exasperated. “Look…I need to know what happened to my husband. One minute he was fine, and the next he was gasping! He had a heart attack I think, er--, I don’ t know, but he was MY husband. Why won’t you tell me?”

The detective looked annoyed. “We haven’t heard back yet from the coroner Mrs. Bleu…but this is an active homicide investigation. We will share that information with you when we get it. Believe me, when we get confirmation of the cause of death, we will be talking again….soon.”

He stepped out. He had heard some good ones, but “he fell on my knife”? Really?? At that moment, the coroner stepped off the elevator and into the empty hallway….she was out of breath and appeared to be sweating.

“Frank! Frank, listen! I just finished the autopsy. It missed!”

“What? What missed? Was it homicide?”

“No! I mean, maybe. Er—I don’t know for sure yet, we need the Tox. results.”

“Toxicology results? …for a stabbing? And what do you mean it missed?”

“That’s just it, the knife! It missed the brachial artery! It was only muscle damage, mostly venous bleeding…seeping blood…comes out slow. It was halfway coagulated before he died and wasn’t even that deep.”

“What? Will you just calm down and start talking in plain English! What are you trying to say?”

“It was a flesh wound…plenty of blood, but not enough to kill a person. Without arterial damage it would take a long time to bleed out from this wound. But there is more to it… this guy died shortly after he was stabbed, but it wasn’t from the knife, that much is for sure!”

The detective cursed under his breath… it was going to be a long day. “Okay, so the guy didn’t die from the knife wound, but he is dead, so from what?!!”

1.2 Introduction (continued)

Figure 1.3

Two weeks have passed and you have been brought in to help investigate this case. You have heard the interrogation tapes and been briefed by both the lead detective and the coroner. The coroner has ruled the cause of death of Dr. John Bleu a homicide, but by poisoning, rather than because of the knife-wound inflicted by Mrs. Bleu, presumably now by accident. Small quantities of an unknown substance, hereafter referred to as Compound X, were discovered in the decaf-coffee Dr. Bleu was drinking when he died. Dr. Bleu had brought the coffee home with him from work as he did everyday according to co-workers and his wife. His wife is still under suspicion, but it has not escaped the notice of the investigators that Dr. Bleu leads a large research group studying potentially lethal enzyme inhibitors for the drug company Hinesbiopharma.

Figure 1.4

The company is working with the US government in a counter-terrorism effort to study the effects of chemical weapons so that potential treatments can be created in advance. Three scientists who Dr. Bleu supervised are running independent projects studying different inhibitors toward the same enzyme, referred to in company documents only as XYZase. XYZase is critical to human metabolism and so development of chemical inhibitors of this enzyme by terrorist groups is viewed as a real and present threat by counter-terrorism experts. The three scientists are Dr. Greene, Dr. Gray, and Dr. Wight. According the company policies, only Dr. Bleu and the single scientist assigned to each project have access to the highly valuable but extremely lethal compounds each one is studying.

To complete the case, you may choose which action you would like to complete next. You will be given enzyme kinetics data, and you will need to use Microsoft EXCEL (or another graphing program) to graph the data in order to determine the kinetic parameters and the type of inhibition. A tutorial to guide you through using MS EXCEL is also provided.

1.3 Investigation Options

Run kinetics experiments on XYZase without inhibitors and with Compound X

Results: The compound found in the coffee (Compound X) has yet to be identified, but using the small amount extracted, you have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that this compound is the most likely murder weapon. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed V0 values given in units of µmol product formed per minute.

[S] V0(No inhibitor) V0 (Murder Weapon - Cmpd. X)
50 0.14 0.040
75 0.19 0.050
150 0.32 0.080
400 0.47 0.14
Table

testtext

true

Question 1.1

MSbtpYzWH7MKswCpnXm7Qq6x2aJSy5WYMlJyzZPnX2yB5x6rg+WLeKwNijZn4uYrG5LsgQRPy956gvdvTNknWJ2kBc0XuuaPDdIPlaTC2QxUDiLjDRa4n2axltIBvUdnPxco3cvq3W64Cq5k0nfUTaaNa35sn7g70d0YULr8huKeUv+uKxCaBND7RRJJk6yDvAj7LQkZjMZWMkrv/QdnBJR6Ip2Ftb0L9r8c3R4kSgxiM5i2cCDmZZ2w110G/CMhf0KbmbWL9A0sFNedD/id4/61mmwsHV9Am/i18EdxzLxHXeNO+cPKbXufyhNXHbPfQqgUUsCG9PmxQoClyLv54L4GutS6Z4/0Y04knMEyJeD9EWhuPscLQYInEszybtO0gzvHT/mEQgwaEs9B1oCfcUHBTMr39EZBAlFamc2xYT4p6sQTeJyBa8j+PbMQbKqve/MTaxw6EU5OoXJLQOBSLYZTQDoOEAHXvz+buTFoQzw=
Correct.
Incorrect.
true

Question 1.2

qmyDrSR6ejaePmRPI1e1+ZOdGjr9E/o0Gx7ZT1ejrZBhLks688xC+86VXEhJwbs8NkcZKHtGclqpXeVAWdqlz2xE9WGFEGJqynrop52NNJDrirV4dWcv1LUUyHBS4qNiKHNTaQ==
3
Correct.
Hint: Refer to pgs. 203-204 of Lehninger 6E; Km can be determined quickly and accurately using a Lineweaver-Burk plot.
Incorrect.
true

Question 1.3

Sample kcat calculation:

In an enzyme assay, you determine the Km for your enzyme to be 0.50 mM. You measure the maximum rate of the reaction to be 0.050 mmoles/second. Calculate kcat for this reaction if the above experiment was conducted with a 1mL reaction volume at an enzyme concentration of 100 nM.

kcat = Vmax/Etotal

[E] = 100 nM

Etotal = [E] x volume

Etotal = (100 nM)(1mL)

Etotal = (1 x 10-7 M/L)(1 x 10-3 L)

Etotal = 1 x 10-10 moles

Vmax = 0.05 mmoles/second

kcat = Vmax/Etotal

kcat = (0.05 mmoles/second) / (1 x 10-10 moles)

kcat = (5 x 10-5 moles/second) / (1 x 10-10 moles)

kcat = 500,000 s-1

Note: In this problem knowing the value of Km was irrelevant…this is true because the value of Vmax was given. If in another problem we were instead given the initial velocity (V0) at some concentration of substrate, then we could use the Michaelis-Menten equation, and the value of Km, to first calculate Vmax !

Also Note: When V0 is given in units of molarity per unit time, then kcat = Vmax/[Et] where [Et] is the concentration of the enzyme in the assay; however, when Vmax is given in moles per unit time, then kcat = Vmax/Et, where Et is the number of moles of Et in the assay.

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
5
Correct.
Hint: Refer to pg. 205 of Lehninger 6E. Be certain that you use correct units in your calculations: kcat should be in units of s-1 and all other units must cancel out in the calculation. Note: when Vmax is given in units of M/min., then kcat = Vmax/[Et] where [Et] is the concentration of the enzyme in the assay; however, when Vmax is given in moles/min, then kcat = Vmax/Et, where Et is the number of moles of Et in the assay. Try your calculation again.
Incorrect.

Given that Dr. Bleu appears to have been killed by an inhibitor to XYZase, you now have sufficient cause to search the labs of Drs. Greene, Gray, and Wight so that you can recover and test samples of the compounds they are each working on developing. This discovery has opened up a new investigation option! New option is:

true

Search the labs and run kinetics experiments on suspicious samples

By gathering samples from the labs of Drs. Wight, Greene, and Gray, you hope to discover which of these people may have had access to what is now clearly the murder weapon, Compound X. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed V0 values given in units of µmol product formed per minute.

[S] V0 (Sample 1) V0 (Sample 2) V0 (Sample 3)
50 0.080 0.067 0.055
75 0.11 0.080 0.063
150 0.19 0.14 0.086
400 0.36 0.24 0.10
Table
true

Question 1.4

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
5
Correct.
Try again.
Incorrect.
true

Question 1.5

njG7KsTQdUNzrEAxcyjg/ZG6swckzbfbGSIGEXddcKJnG9juT9xsw5ABZyo7mNIAPFth3N1AAQ/3Pt3YqkaAMcFHJv6wojDIMVM+XigZaQNVZoKcSHKF9vesKLp9eU6izSEsLvyYDOQgbrOB2FIC8vnHEHe4r/COaJ4JfxRARkLMdfXl0DcF9LeSVUaiISHDTdDnvqxoD6E1yFQKXgoShldsgHVG1tY2d1IhCWfQUKxAXEcKiRz6yZBF0Nk28ggQA4KpzIE+QynQtuG5Je5Yt9xZ+9LBw9S/CeoW9i4lV3j4YjlsmQUY5m3b+7wNn8HsNDJwcHLKY7gAGesQ4nAlYYbbmEF5Z3MRiFqv/fPg6HVd2psTHVxhZ8G0/UYsOLdOQ9ECIfowEdp3NQCmqUCMXL2+LX8Edn0z7/g1LHYqfhmTG9uP1qRI3tO4djCGgb7PRfoU94vLqnpgHcIM
Correct.
Incorrect.
Hint if one or more are matched incorrectly: You have incorrectly matched one or more samples. Please reexamine your data and review pgs. 207-211 of Lehninger 6E & try again

You notice, however, that the samples you collected from the labs are now labeled 1, 2 and 3. You ask your lab assistants which samples correspond to which labs, but they mixed up the samples (somebody didn’t label the tubes when running the assays!). This is a disaster! Now, in addition to determining which sample (if any) matches the murder weapon, you are going to have to testify in court as a biochemist to convince a jury that the guilty sample (1, 2, or 3) belongs to the guilty person (Drs. Greene, Wight, or Gray) based on what you know about enzyme kinetics! Are you up to the challenge? Who killed Dr. Bleu?

The good news is that you may now go back to Hinesbiopharma and interview the three scientists about the molecular mechanism of the inhibitor each is working with. This has opened three new investigation options which are:

Interview Dr. Greene

Results: Dr. Greene is studying a compound that binds noncovalently to an allosteric site on the protein approximately 20Å away from the active site. She has solved crystal structures, which have revealed that this allosteric site is blocked in the absence of substrate; a tryptophan residue blocks the ligand-binding pocket. When the enzyme binds the substrate, a shift in a helix connecting the active site and the allosteric site displaces the Trp sidechain, clearing the pocket and allowing the inhibitor to bind. When the inhibitor is bound, it prevents a nearby loop from moving to associate with the remainder of the active site. The association of this loop with the active site is necessary for catalysis to occur. Binding studies have confirmed predictions made by the crystal structures: in the absence of substrate, the inhibitor binds with a Kd of 10 mM, but in the presence of the substrate, the inhibitor Kd is decreased to 1 nM.

true

Question 1.6

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
4
Correct.
Try again.
Incorrect.

Interview Dr. Gray

Results: Dr. Gray is studying the effects of a transition-state analog. This molecule, which is a fluorinated lactone called F-lac by the company, mimics the geometry of the theoretical transition state in the XYZase reaction mechanism. Dr. Gray has solved crystal structures that have confirmed that F-lac binds noncovalently in the active site as expected. This result triggers the following assessment question:

true

Question 1.7

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
4
Correct.
Try again.
Incorrect.

Interview Dr. Wight

Results: Dr. Wight works on an inhibitor of XYZase that acts by binding to a lysine residue in the enzyme active site, forming a Schiff-base. This lysine is critical to function, normally acting as an acid in the enzyme mechanism. Stability studies have confirmed that under physiological conditions, the inhibitor- Schiff-base linkage formed in the enzyme active site is extremely stable, with a half-life >of more than 24 hours.

true

Question 1.8

zN4T6fz1r72VpWsQqoXfIadxYdPqCwue58i6hqzlL5p+lvVtdpYFdIYSh2evE3BzMHVAe76WXk9ljP+/IbFnm1RXJ3wyEg1P96bqViYEVFcLCrX7Z1sQ0AY783VVS7ZfvGdf9Zqe2+XlqNMXvt+f71XDpvZrDi8CkT1HZDNXhOClh0t+mxpGrfkC7ai+22l9wyuSUqW9UZgDehjqkp3chiF+OOm8GJSNiXvILWQo7Wk2sQ3JYoBO73THxR13AD7vhFOwcGEQ7NWFhXIgrOg4aBIbm9WUEQTdOnHto1dBsxXmz/QDXcvvse67jSEOy0315ZpFQOFweQk3k2KNztoLq6qnk1X9m+YqWgZTKjUn3gzKczprpdOLCoNfK2dLwt1I13yOjstNQJ1y229jjPsfoKM+iRlGLHLWmsPSA8NxVgKXHY+RBoWmu/+Mr+6N9t37oCiSaM9G57q2TcjnMlBj/Pgh7m4f6dpUVAHPLz6S91KKLtypQlyvi+iU1YmQmI/I6RiEpnLm7DwQQwnASvEzHMP6bEL01WEmirFkwZ580kznqUdbY5hsDa9ZbWV3N+kCxWPFDX4O9A4/O1r7nQ/e8sBnh0QMWSwspmHi8i0B/OVKatZsWme63TrO8dr7JYJWAfjiA9Qk4y+n/XSGQ+aWUS8yxTPLwWybDI3rwfxamqAog9WbnPsHCZasimnroX9IeKjxFzc8g3jYIrZEmw8+lQq+OGUEnihnC5cDPFuMm1a7KiAKiTpmWdlP4W9xW5JUsgpBUJOkJLsNU/R8Quw5PM1kOeaS8P0g5A7zlHpSyYzouRvBViW0nBvmbz9bIGh/1sIwyjOVTwyN9YT9EGUUCutU2n946fNYnWZtYA9kKKsFf/mGGqGPVVOcgoavaYTSmqOluVqxECkO6Mz20zBDHguXy4Sr6NoReld5/VOSQKju7cV3
3
Correct.
Try again.
Incorrect.
true

Question 1.9

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
3
Correct.
Try again.
Incorrect.

1.4 Assessment

You have now completed the investigations and can go on to answer the final assessment questions:

inv121
true

Question 1.10

Based on the information you have gathered, and your answers to previous questions, match the samples to their respective scientists. Hint: review the information from interviews and answers to previously answered questions.

lFQzc38hOsyuOWYk063X057tds2dYgc81tuyKAPfDbw4yNL7ynWVc8cQ3XNqsqVFPABGe+SlXLmFQmpTEZdCBhA2zM4tGIzq
4
Hint if one or more are matched incorrectly: You have incorrectly matched one or more of the samples. Please reexamine your data and review pgs. 207-211 of Lehninger 6E & try again
Correct.
Incorrect.
true
inv109
true

Question 1.11

z3i7/RUz06qf/nOhb/eD62qg0fNWoT5jcqQnH7YoTDiySlEUJac2V3g7hdI11z6ZKpe9OTINoGSn/GMIeiZn/leh7yKPi6N/b9GsF3JUuTbfB1u+IugfFPgaINdxY8/aMGHlnZ3SkDQa5oVCdA8O5hrCRgpM4TEd0lyGGjr/o9hMTSVAmuDnycSy2wyf9jOmzwiAK9jVqQ8EZ2OrSshY8TkNGnu7GIEt/mLQIkNZ5ShWTz5Dh/bJI+8kSUyyqxE5EEmVua2e2+Q1xlSwSl3wKd8X5F/v9bXWKPpyrcqfMg9HC0u0D628w7XJyj2L0nLs+f+Fcg5enNK9C/+BPjKv6Ok5lsUVQ29pxkf+zJ/gQmPsXTC4uZHd8m8czVpkbzEPfcAR6eG9ZQAxMAEkj1mDSS5hGbYub8I5aNGAWtXgfrwtiEmau4gUusmbcDzr9rGZDKJverNAR4UtlBhLVMEPYYghMnYDDDKp3jewUH2F8kyEX9w7r97V8+YZt2LYRKwezOxVxUhnjT8Aq0q7zXkvC6V1T9YbGwot31EBtngRIbAG2OXX7M3GTbOTx4PPbEVVpezDx6Gb8c+NnWPmTMXR4eFLmAu0tQVyjqavRKotCiWgGIGGN6rB7Bw9vKpS0KjRE3DErTgIIX+FzU5bmfv87LpzDfxR5D4GkEmESNzK8O6jKc0hi+GgwpGCVVtYSvSLQPTvk1AIV1KoMCR0Ox/51Qoe2/EYPzHZsGGzW8owzlNVowvrt91L2eb7HINZfM+S8whYPsMGy5q65Q7QbNEMqimdNqSvgY5FTE4cZu5CdUHo8HdaZpkbdDRt9y+PqczPcAQFOfR8U0oUnUj46XziHbd590ojaTXRPc/ELqRKwHjRfY6sO9lbmmyNQ7oTY2szv6GRi3Z78xGPKHKdcAh/03KINNshzYNUFzV6K6J5oxERurNkRcjmK3nogod2DlZMQ1O0ZzQO3YafV+qxMPmIfCXKODgkg8SWkgg4cbKh3vDfg8E787mkd2EzxqgrTsoeos96B6u5F0wvTCHl
4
Correct.
Incorrect.
true
inv110
true

Question 1.12

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
2
Correct.
Incorrect.
true
inv111
true

Question 1.13

During their three-week pre-marathon self-experiment, both Dave’s and Michael’s livers had to produce some glucose. Which of the following molecules could be used by the liver to create the glucose that is needed to supply the brain and other tissues in a healthy person on a normal diet? (Mark "yes" for ALL that apply!)

aiSHEd13EbnkbSzqJrX9DBXWq8pPLYWEV0S4rV9kqYX0VKCH2xFlYcyQpzFVNX7kTRw8X1gFMHlF+sJwVLbZGXjjvF0riAilqs8qmEjqDHXoBlHFg3+bErBCcixwGYxKB28ccJWHVBbVWdEZxnHN8ON3Dpymb8Fqj5vfiw4RimkOmgkdktgwiR4poaJLV3qGbC7QU86QXfUtBWZsIlQjMVw7JonVC4236RSySVjE7WdhkkOX/KMvPocZ8mDfpYWP32weHzEpZlyNtB+WY46xw49UYo7jbj7D8w/gtA==
2
Correct.
Incorrect.
true

Activity results are being submitted...

1.5 Case Summary

Congratulations on completing this Case Study! The following Case Summary gives a full explanations of the murder of Dr. John Bleu.

Case Summary

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi in gravida orci. Cras elementum nulla a lacus ornare ultricies. Aliquam mattis purus at libero ultricies consequat. Nunc at arcu nibh. Vestibulum in imperdiet turpis, vel vehicula nunc. Donec sagittis justo scelerisque massa ornare, eget vestibulum turpis ornare. Donec pulvinar sodales tellus. Suspendisse potenti. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Cras tempus ligula ac odio mollis, sed vulputate tortor tincidunt. Duis ligula tellus, fringilla id mollis in, sollicitudin at augue. Nullam vitae accumsan diam. Donec ac congue turpis, vel auctor lectus. Praesent egestas blandit ante, vel placerat purus blandit ac.

Donec lacinia elit eros. Praesent scelerisque ipsum arcu, at semper nunc imperdiet id. Duis ac odio augue. Nulla eu volutpat odio, id gravida enim. Nam sed neque nec erat pretium vulputate eget quis elit. Phasellus faucibus dolor diam, ut ultricies lacus porta sit amet. Praesent odio sem, aliquet et tristique quis, tristique nec mi.

Pellentesque scelerisque tincidunt vehicula. Vivamus dignissim cursus malesuada. Nullam consequat elit id vulputate facilisis. Sed tincidunt magna non commodo suscipit. Donec volutpat libero nisi, eu molestie lectus elementum a. Ut a leo mi. Ut vehicula elementum metus id accumsan. Sed ut nisi ipsum. Aenean vel est sit amet arcu porttitor convallis. Etiam aliquam ex orci, sit amet convallis leo suscipit at. Mauris eget felis non nisi consequat rhoncus non id nisi. Pellentesque at hendrerit ex. Duis mi risus, finibus quis nunc quis, accumsan iaculis ante. Etiam eleifend, libero blandit ultrices sagittis, nulla dui semper nunc, a sagittis nibh neque ut purus. Phasellus laoreet sed augue volutpat molestie.

Praesent cursus, neque vitae consequat sollicitudin, quam purus dapibus nunc, vitae volutpat magna sem id turpis. Vestibulum eros dolor, accumsan lacinia sagittis et, tincidunt at justo. Suspendisse magna lacus, pretium non posuere quis, pellentesque vitae risus. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Praesent dignissim eros in tellus tristique, sed luctus nisl lobortis. Quisque euismod pharetra ex placerat scelerisque. Nunc porta malesuada quam eu tempus. Fusce lectus enim, consectetur sed dolor eget, pellentesque porttitor velit. Nullam consectetur a erat quis tristique. Morbi euismod consectetur consectetur. Maecenas viverra finibus pulvinar. Interdum et malesuada fames ac ante ipsum primis in faucibus. Suspendisse ut purus lacus.

Aliquam lectus ante, hendrerit in ornare sit amet, vehicula et velit. Mauris id aliquam sapien. Fusce at nunc nibh. Aliquam eu velit dictum, auctor nisi non, imperdiet ipsum. Nulla sed arcu hendrerit, sollicitudin tellus sed, rutrum tellus. Duis ornare lorem sit amet vestibulum imperdiet. Donec varius augue et libero molestie, in eleifend orci mollis. Aenean libero leo, pellentesque eget justo eu, tristique porta ligula. Fusce scelerisque varius ipsum. Pellentesque suscipit fringilla consectetur. Quisque lacinia lorem quis commodo blandit.