The application of road salt in cold climates can result in runoff pollution that can damage aquatic ecosystems. Researchers at Saint Mary’s University in Halifax surveyed local roadside ponds (some were occupied by amphibians and some were not) to determine chloride concentration and species richness of amphibian species. They also investigated the vulnerability of 5 amphibian species to sodium chloride (NaCl) by determining the LD-50, the dose or concentration of chloride that kills 50% of the population.
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