We conclude this chapter with a brief discussion of transcription initiation by the other two eukaryotic nuclear RNA polymerases, Pol I and Pol III. The distinct polymerases that transcribe mitochondrial and chloroplast DNA will be discussed in Chapter 12, on cellular energetics. Although these systems, and particularly their regulation, are less thoroughly understood than transcription by RNA polymerase II, they are equally fundamental to the life of eukaryotic cells.