key concept 18.1 DNA from Different Sources Forms Recombinant DNA
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key concept 18.2 There Are Several Ways to Insert DNA into Cells
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Selectable genetic markers are used to identify host cells containing recombinant DNA
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Genes can be inserted into prokaryotic or eukaryotic cells
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Inserted DNA is usually integrated into the host chromosome
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Reporter genes help select or identify host cells containing recombinant DNA
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key concept 18.3 Any Sequence of DNA Can Be Used for Cloning
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DNA for cloning can come from a library
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cDNA is made from mRNA transcripts
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Synthetic DNA can be made by PCR or by organic chemistry
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key concept 18.4 Several Tools Are Used to Modify DNA and Study Its Function
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Gene expression can be modulated by DNA technology
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DNA mutations can be created in the laboratory
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Genes can be inactivated and changed by CRISPR technology
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Complementary RNA can prevent the expression of specific genes
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DNA microarrays reveal RNA expression patterns
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key concept 18.5 DNA Can Be Manipulated for Human Benefit
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Cells can be turned into factories for a desired protein
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Medically useful proteins can be made using biotechnology
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DNA manipulation is changing agriculture
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Synthetic biology can create living factories for new products
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There is public concern about biotechnology
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Apply What Youâve Learned
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