Photosynthesis, the means of converting light into chemical energy, takes place in organelles called chloroplasts, typically 5 μm long. Like a mitochondrion, a chloroplast has an outer membrane and an inner membrane, with an intervening intermembrane space (Figure 19.2). The inner membrane surrounds a space called the stroma, which is the site of the dark reactions of photosynthesis (Section 20.1). In the stroma are membranous structures called thylakoids, which are flattened sacs, or discs. The thylakoid sacs are stacked to form a granum. Different grana are linked by regions of thylakoid membrane called stroma lamellae (Figure 19.3). The thylakoid membranes separate the thylakoid space from the stroma space. Thus, chloroplasts have three different membranes (outer, inner, and thylakoid membranes) and three separate spaces (intermembrane, stroma, and thylakoid spaces). In developing chloroplasts, thylakoids arise from budding of the inner membrane, and so they are analogous to the mitochondrial cristae. Like the mitochondrial cristae, they are the site of coupled oxidation–reduction reactions of the light reactions that generate the proton-motive force.

The thylakoid membranes contain the energy-transforming machinery: light-harvesting proteins, reaction centers, electron-transport chains, and ATP synthase. These membranes contain nearly equal amounts of lipids and proteins. The lipid composition is highly distinctive: about 75% of the total lipids are galactolipids and 10% are sulfolipids, whereas only 10% are phospholipids. The thylakoid membrane and the inner membrane, like the inner mitochondrial membrane, are impermeable to most molecules and ions. The outer membrane of a chloroplast, like that of a mitochondrion, is highly permeable to small molecules and ions. The stroma contains the soluble enzymes that utilize the NADPH and ATP synthesized by the thylakoids to convert CO₂ into sugar. Plant-leaf cells contain between 1 and 100 chloroplasts, depending on the species, cell type, and growth conditions.

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Chloroplasts contain their own DNA and the machinery for replicating and expressing it. However, chloroplasts are not autonomous: they also contain many proteins encoded by nuclear DNA. How did the intriguing relation between the cell and its chloroplasts develop? We now believe that, in a manner analogous to the evolution of mitochondria (Section 18.1), chloroplasts are the result of endosymbiotic events in which a photosynthetic microorganism, most likely an ancestor of a cyanobacterium (Figure 19.4), was engulfed by a eukaryotic host. Evidence suggests that chloroplasts in higher plants and green algae are derived from a single endosymbiotic event, whereas those in red and brown algae are derived from at least one additional event.

The chloroplast genome is smaller than that of a cyanobacterium, but the two genomes have key features in common. Both are circular and have a single start site for DNA replication, and the genes of both are arranged in operons—sequences of functionally related genes under common control (Chapter 31). In the course of evolution, many of the genes of the chloroplast ancestor were transferred to the plant cell’s nucleus or, in some cases, lost entirely, thus establishing a fully dependent relation.